Name: operon control by activators and repressors
Uploaded: 2020-11-23T11:47:37.000Z
Duration: 1 min 58 s
Channel: JoVE

原核生物は環状ゲノムを含んでおり、多くの場合、特定の生化学的プロセスに関連する遺伝子がDNA配列内で互いに直接隣接して位置しています。 
オペロンとして知られるこれらの遺伝子クラスターは、単一のプロモーターを持ち、単一のmRNAに転写されます。オペロンの発現は、それぞれ転写を促進または抑制するタンパク質であるアクチベーターとリプレッサーによって制御されます。 
活性化剤はプロモーターに結合し、RNAポリメラーゼの結合とそれに続くオペロンの転写につながります。
リプレッサーは、オペレーターと呼ばれるプロモーターの近くにある異なるDNA配列と相互作用します。リプレッサーがオペレーターに結合すると、RNAポリメラーゼがプロモーターに結合するのを防ぎ、転写を阻害します。
原核生物の遺伝子調節は、多くの場合、栄養素の利用可能性に依存します。一部の細菌では、グルコース濃度が低いと、サイクリックAMPが蓄積します。 
サイクリックAMPは、活性化剤である異化活性タンパク質に結合し、それらが一緒になってプロモーターに結合します。これにより、細菌がグルコース以外の糖を代謝することを可能にする遺伝子を含むオペロンであるlacオペロンの発現が開始されます。
トリプトファンは、タンパク質の合成に必要なアミノ酸です。トリプトファンが環境から入手できない場合でも、一部の生物は自分自身でトリプトファンを生成することができます。その合成のための遺伝子は、リプレッサーによって調節されているオペロンに存在します。
トリプトファン濃度が高い場合、トリプトファンはリプレッサーと会合します。この複合体はオペレーターに結合し、RNAポリメラーゼをブロックし、転写を抑制します。

<ol>
	<li>Alberts et al., 6th edition; pages 380-383</li> (英語)
	<li>Shaw、K.(2008)原核生物における負の転写調節。自然教育 1(1):122</li>
</ol>

原核生物の転写活性化因子および転写抑制因子

Prokaryotic Transcriptional Activators and Repressors

ゲノム内の原核生物の遺伝子の構成は、真核生物の構成とは著しく異なります。原核生物の遺伝子は、同じ生化学プロセスまたは機能に関与するタンパク質の遺伝子がグループとして一緒に位置するように編成されています。この遺伝子グループは、その調節要素とともに総称してオペロンとして知られています。オペロン内の機能遺伝子は一緒に転写されて、ポリシストロニックmRNAとして知られる1本鎖のmRNAを形成します。

オペロンにおける原核生物の遺伝子の転写は、アクチベーターとリプレッサーとして知られる 2 種類の DNA 結合タンパク質によって制御されます。アクティベーターは、転写開始部位であるプロモーターに結合し、転写に関与する重要な酵素であるRNAポリメラーゼの結合を助けます。リプレッサーは、プロモーターと遺伝子の間のオペロン内の短い調節配列であるオペレーターに結合し、RNAポリメラーゼのプロモーターへの結合を阻害します。

アクチベーターとプロモーターの構造上の前提条件は、DNAに結合できる構造と結合できない構造の 2 つの交互構造で存在できる必要があることです。アクティベーターに特有のもう1つの特徴は、RNAポリメラーゼとDNAの両方に同時に結合する2つの結合表面を持っていることです。 この2つの分子の動員により、ポリメラーゼがプロモーターに近づき、その結合が促進されます。アクティベーターには転写において触媒的な役割はなく、その機能は酵素とDNAの結合を促進することに限定されています。 活性化因子が存在しない場合でも、RNAポリメラーゼはDNAに結合し、低レベルの発現を示す可能性があります。 このシステムにリプレッサーが存在すると、その遺伝子の基礎的な発現が妨げられます。

原核生物の遺伝子発現の制御は、栄養素の利用可能性と生物の要件に大きく依存します。これらの栄養素は、アクチベーターとリプレッサーのオペロンへの結合を制御し、必要な遺伝子セットのみが確実に発現されるようにします。たとえば、細胞内にトリプトファンが存在すると、トリプトファンがリプレッサーに結合し、trpオペロンの転写とその後のトリプトファンの生成が妨げられます。

原核生物の遺伝子のゲノム内の構成は、真核生物のそれとは著しく異なります。原核生物の遺伝子は、遺伝子が...

prokaryotic-transcriptional-activators-and-repressors

Education

JoVE Core

Molecular Biology

分子生物学

Unit 1

ユニット1

Gene Expression

遺伝子発現

KEY TERMS AND CONCEPTS

主要用語と概念

operon control by activators and repressors

Operon control in prokaryotes depends on activators and repressors. Prokaryotic genes are often arranged in groups, not one by one like in eukaryotes. Genes that help carry out the same biochemical process are placed together with their regulatory elements. This group is called an operon, and it is transcribed into a single polycistronic mRNA strand.

Activators and repressors are DNA-binding proteins that control transcription in an operon. Activators bind near the promoter, which is the site where transcription begins. They help RNA polymerase, the enzyme that makes RNA, bind to the promoter. Repressors bind to operators, short regulatory sequences between the promoter and the genes, and they block RNA polymerase from binding to the promoter.

These proteins must switch between two shapes. One shape allows them to bind DNA, and the other does not. Activators also have two binding surfaces. One surface binds DNA, and the other binds RNA polymerase at the same time. This brings the polymerase closer to the promoter and helps transcription start. Activators do not catalyze transcription. Their role is to help the enzyme and DNA come together.

Without an activator, RNA polymerase can still bind to DNA, but only at low levels. If a repressor is present, even this basal expression is prevented. In prokaryotes, gene expression is largely controlled by nutrient availability and the needs of the cell. These nutrients affect whether activators and repressors bind to the operon, so only the needed genes are expressed. For example, when tryptophan is present in a cell, it binds to a repressor and stops transcription of the trp operon. This also prevents more tryptophan from being made.

Operon Control by Activators and Repressors

operon-control-by-activators-and-repressors

cell specific gene expression

How Cells Switch Genes On and Off

Cells switch genes on and off to make different proteins for different jobs. In multicellular organisms, many cell types are built from the same DNA, but they do not use the same genes in the same way. This difference in gene expression helps explain why cells can look and act so differently. Liver cells are a clear example. They help detoxify blood, make bile to help digest fats, and produce proteins needed for metabolism. To do these jobs, liver cells must express a specific set of genes.

regulation of expression occurs at multiple steps

regulation-of-expression-occurs-at-multiple-steps

How Cells Control Gene Expression

Cells control gene expression at several points between DNA and protein. The most common control point is transcription. At this stage, proteins bind to short regulatory DNA sequences and can either increase or reduce transcription of the linked gene. After transcription, a precursor mRNA is made. This pre-mRNA contains exons, which code for protein, and introns, which do not. It must be processed before translation, and splicing removes the introns and joins the exons. Cells can also regulate...

cis-regulatory sequences

How Cis-Regulatory DNA Controls Transcription

Cis-regulatory sequences are short, non-coding DNA segments that control gene transcription. They sit on the same chromosome as the genes they regulate and act as binding sites for transcriptional regulators. In eukaryotes, these proteins help control gene expression patterns in different cell types. These DNA sequences can be close to a gene or thousands of bases away. When they are far from the gene in the DNA sequence, they are often brought close together in space because chromosomes are...

cooperative binding of transcription regulators

cooperative-binding-of-transcription-regulators

How Transcription Factors Bind DNA

Transcriptional regulators bind to cis-regulatory DNA sequences to control gene transcription. These DNA sequences are very short, usually fewer than ten nucleotide pairs long. Because they are so short, the same sequence can appear by chance in many places across the genome. Some regulators can also bind to groups of similar sequences. That raises the chance of random binding even more. To lower this risk, transcription regulators often form dimers, which are pairs of proteins that bind a DNA...

prokaryotic transcriptional activators and repressors

Operon control in prokaryotes depends on activators and repressors. Prokaryotic genes are often arranged in groups, not one by one like in eukaryotes. Genes that help carry out the same biochemical process are placed together with their regulatory elements. This group is called an operon, and it is transcribed into a single polycistronic mRNA strand. Activators and repressors are DNA-binding proteins that control transcription in an operon. Activators bind near the promoter, which is the site...

operons

Operons in Bacterial Gene Control

Operons help bacteria control groups of genes at the same time. An operon is a set of genes that are regulated together and can be turned on or off as a unit. This shared control helps bacteria respond quickly to changes in their environment. In many operons, a promoter gives RNA polymerase a place to bind and start transcription. The operon also includes a regulatory region that helps control whether the genes are expressed. When conditions change, the cell can use this setup to manage gene...

the eukaryotic promoter region

Eukaryotic Promoter Motifs and Gene Control

The eukaryotic promoter region is a stretch of DNA located upstream of a gene. It includes an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. These DNA signals help control when transcription begins. The promoter can be divided into proximal and distal regions. The proximal promoter lies close to the gene and includes cis-regulatory sequences and the core promoter. The core promoter is the RNA polymerase binding site, and it is usually found...

co-activators and co-repressors

Chromatin Control in Gene Transcription

Chromatin control plays a major role in gene transcription in eukaryotes. Gene expression is regulated by several proteins that work together at a gene regulatory site. Some of these proteins bind directly to specific DNA sequences, while co-regulators attach to those regulators but do not bind DNA on their own. Co-regulators can act as co-activators or co-repressors, depending on the complex they join. One co-regulator may even switch roles in different settings. For example, the...

eukaryotic transcription activators

How Transcription Activators Turn Genes On

Transcription activators are proteins that help turn genes on by promoting transcription from DNA to RNA. They often have two parts. One part binds to DNA, and the other part activates transcription. In some proteins, such as the glucocorticoid receptor and MyoD, one domain can do both jobs. The DNA-binding part recognizes specific regulatory sequences on the DNA. These binding domains are grouped into families based on their structure. Common examples include the leucine zipper, zinc finger,...

eukaryotic transcription inhibitors

How Eukaryotic Gene Repressors Work

Eukaryotic gene repressors help turn specific genes off when the cell needs to control growth, development, or other biochemical processes. These DNA-binding proteins regulate gene expression in eukaryotes. Their presence at the right time and place can be triggered by hormones and signals from other cells. Most transcription inhibitors have two main parts. One part binds DNA, and the other is a repressor domain, which helps shut down transcription. Repressor domains can connect with basal...

combinatorial gene control

Gene Expression by Transcription Factor Combinations

Gene expression can depend on combinations of transcription factors working together. In combinatorial gene control, several transcription factors act in a coordinated way to regulate a single gene. If one or more of these factors are missing, the level of gene expression or repression can change a lot. This kind of control helps explain how a relatively small number of transcription factors can regulate a very large number of genes. More than 30,000 genes are controlled by about 2,000 to...

induced pluripotent stem cells

Reprogramming Cells into Stem Cells

Induced pluripotent stem cells are created by reprogramming mature cells back into a stem-cell state. This process shows that specialized cells can be changed into cells with broader developmental potential. The topic is important in biology because it links cell identity, gene control, and stem cell research. These cells begin as ordinary body cells and are pushed to de-differentiate, or lose their specialized features. After reprogramming, they behave like pluripotent stem cells, which means...

master transcription regulators

MEF2C and Gene Control Cascades

MEF2C is a master transcription regulator that controls gene expression in coordinated cascades. These regulators are proteins that manage the activity of multiple genes that often work together to produce a complex outcome. When a master transcription regulator is activated, it can start a chain of transcriptional activation that supports that outcome. Master transcription regulators can act in two main ways. They may bind directly to the regulatory sequences of several genes involved in a...

epigenetic regulation

DNA Methylation and Histone Changes

Epigenetic regulation controls gene activity without changing the DNA sequence. It helps determine when genes are turned on or off in a cell. Two major epigenetic mechanisms are DNA methylation and histone modification. DNA methylation adds chemical groups to DNA, while histone changes affect how tightly DNA is packed around histone proteins. These changes can make genes easier or harder to use. In high school biology, epigenetic regulation is important for understanding how cells with the...

genomic imprinting and inheritance

Genomic Imprinting in Human Disease

Genomic imprinting affects how some inherited genes are turned on or off in human cells. Diploid organisms inherit chromosomes from both parents, and each gene usually has two alleles. In most cases, both alleles are expressed. If one allele is missing or mutated, the other can often compensate, but some genes do not work that way. In genomic imprinting, a gene or its regulatory sequence carries chemical tags, such as methyl groups, on the copy inherited from only one parent. These tags are...

key terms and concepts

Learn how activators and repressors control prokaryotic operons, from promoter binding to the trp operon response to tryptophan in cells.

Operon Control by Activators and Repressors | JoVE K12

molecular biology

JoVE Core 分子生物学は、明確で簡潔なアニメーション ビデオ レッスンを通じて細胞の分子プロセスを紹介します。さらに、科学者の実際のビデオでは、これらの概念の応用が現代の研究室での実際の実験を通じて実証されています。

Operon Control by Activators and Repressors

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