Follicle Vitrification
Follicle Vitrification - Related Videos
10:31
Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle Cryopreservation
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Cited by 3 •
2025
This protocol describes a novel three-dimensional (3-D) culture model using a tyramine-linked hyaluronan hydrogel to encapsulate and culture preantral follicles from the mouse ovary. We also detail two approaches to ovarian follicle cryopreservation by vitrification.
10:41
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
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Cited by 17 •
2015
This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.
06:00
Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
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Cited by 17 •
2011
An ethylene glycol-based vitrification method for mouse embryos is described. It is advantageous to other methods in its simplicity and low embryonic toxicity, and therefore can be broadly applicable to many strains of mice, including inbred and gene-modified mice.
10:48
In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
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Cited by 9 •
2017
A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.
08:44
The Utility of Stage-specific Mid-to-late Drosophila Follicle Isolation
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Cited by 9 •
2013
Stage-specific isolation of mid-to-late Drosophila follicles is useful for a variety of purposes. Such follicles develop in culture, which allows for genetic and/or pharmacologic manipulations to be coupled with in vitro development assays and live imaging. Additionally, follicles can be used for molecular studies, such as isolating mRNA and protein.
FAQs
They act as protective solutes during cooling by replacing or managing cellular water and helping the sample reach a glass-like state rather than forming damaging ice. Their high concentration is important because vitrification depends on rapid solidification without crystallization. In follicle preservation, this protection helps retain the follicle’s organization for subsequent warming and biological assessment.
Extremely rapid cooling limits the time available for water molecules to organize into ice crystals. That matters because crystallization can damage cellular structures within the follicle. Instead, the cryoprotective solution and cooling conditions promote a glass-like state. The result is preservation focused on reducing physical injury during storage, rather than allowing conventional ice formation.
Maintaining follicular structure is important because the follicle is not merely a container for an immature oocyte. Its organization and cellular interactions provide a model for examining folliculogenesis and ovarian function. Preserving those relationships allows researchers to study how follicles survive, grow, and support oocyte development after warming, rather than evaluating isolated cells alone.
After warming, follicles can be placed in culture so their biological performance can be examined. Researchers may assess whether the follicles survive, continue growing, and support oocyte development. These observations provide functional information that is not available from storage alone, helping connect the preservation step with studies of folliculogenesis, ovarian function, and reproductive technologies.
The technique provides a potential way to store ovarian follicles for later use when researchers or fertility-preservation programs need access to reproductive material. Its value is especially relevant when conventional tissue preservation is unsuitable. Subsequent warming and culture can help determine whether preserved follicles remain viable and retain properties useful for reproductive research or fertility-related applications.
Preserved follicles can serve as controlled models for investigating ovarian biology after storage and warming. Because the method aims to retain follicular organization and cellular interactions, researchers can examine processes connected with folliculogenesis, ovarian function, and oocyte development. This makes the approach useful for studying reproductive technologies while keeping the follicle’s multicellular context intact.