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In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
JoVE Journal
Developmental Biology
Author Produced
This content is Free Access.
JoVE Journal Developmental Biology
In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
DOI:

10:48 min

December 17, 2017

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Chapters

  • 00:00Title
  • 01:15Follicle Isolation and Encapsulation
  • 04:29In Vitro Culture of Encapsulated Follicles Under Simulated Microgravity Treatment
  • 08:28Changing Culture Medium
  • 09:17Follicle Retrieval
  • 09:49Results
  • 10:24Conclusion

Summary

Automatic Translation

A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.

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