Insulin Secretion Analysis
Insulin Secretion Analysis - Related Videos
05:51
Coculture Analysis of Extracellular Protein Interactions Affecting Insulin Secretion by Pancreatic Beta Cells
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Cited by 3 •
2013
Transcellular protein interactions are important determinants of pancreatic beta-cell function. Detailed here is a method—adapted from a coculture model of synaptogenesis—for investigating how specific transmembrane proteins influence insulin secretion. Transfected HEK293 cells express proteins of interest; beta cells do not need to be transfected or otherwise directly perturbed.
04:41
Glucose-Stimulated Insulin Secretion via Perfusion through the Mice Vasculature with an Intact Pancreas
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2025
Here, we provide details on performing glucose-stimulated insulin secretion (GSIS) via perfusion through the mice vasculature with an intact pancreas as an additional technique to assess the endocrine pancreatic function with reduction of possible confounders.
05:58
Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate
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Cited by 7 •
2019
This protocol describes how to perform rapid low-cost luciferase assays at medium-throughput using an insulin-linked Gaussia luciferase as a proxy for insulin secretion from beta cells. The assay can be performed with most luminescence plate readers and multichannel pipettes.
08:45
Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
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Cited by 9 •
2016
Here, a new method that allows the conversion of adult skin fibroblasts into insulin-secreting cells is presented. This technique is based on epigenetic conversion, does not involve the use of retroviral vectors nor the acquisition of a stable pluripotent state. It is therefore highly promising for translational medicine applications.
01:27
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
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2024
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell. Insulin and C-peptide are co-secreted in...
FAQs
Elevated glucose increases ATP production inside pancreatic beta cells. The higher ATP level closes ATP-sensitive potassium channels, reducing potassium movement across the membrane and causing membrane depolarization. This electrical change is essential because it links glucose metabolism to activation of voltage-gated calcium channels, providing the signal that initiates insulin release for measurement.
Membrane depolarization opens voltage-gated calcium channels, allowing calcium to enter beta cells. The resulting rise in intracellular calcium triggers exocytosis, the fusion of insulin-containing granules with the cell membrane and their release outside the cell. Measuring secretion therefore reflects the outcome of a glucose-to-calcium signaling pathway, not glucose exposure alone.
Immunoassays quantify insulin in collected samples, allowing researchers to compare release between experimental conditions. Dynamic secretion measurements emphasize how release changes over time rather than only the amount detected at a single point. Using either approach, or comparing their results, can help characterize insulin responses and evaluate beta-cell function under specified conditions.
A typical analysis exposes pancreatic beta cells, tissue, or an organism to a glucose condition and then assesses the insulin released. Researchers quantify that release with an immunoassay or follow it using a dynamic secretion measurement. Comparing the resulting responses across conditions provides a basis for evaluating how glucose affects insulin output.
This analysis is useful when investigators need to examine glucose regulation or beta-cell function. It supports research on diabetes, metabolic physiology, and beta-cell biology, as well as studies of therapeutic compounds that alter insulin release. The same general approach can be applied to cells, tissues, or organisms, depending on the biological question.
Differences in insulin output can indicate that a condition changes glucose responsiveness or beta-cell function. Researchers may compare responses produced by different glucose conditions or by compounds intended to alter secretion. Interpreting these changes alongside the underlying ATP, potassium-channel, membrane, and calcium-signaling sequence helps connect the measured outcome with beta-cell physiology.