Water Droplets

Water droplets are small, discrete volumes of liquid water whose shape and behavior arise from molecular cohesion and interactions with surrounding surfaces. Surface tension pulls water molecules toward one another, while adhesion, gravity, evaporation, and contact with air or biological materials determine whether a droplet spreads, beads, moves, or changes size. In biology, water droplets provide useful models for studying wetting, evaporation, pathogen and biomolecule transport, and compartmentalization in cells. Their controlled formation in microfluidic devices also supports miniaturized assays, cell manipulation, and biological analyses, making droplet behavior relevant to laboratory methods and natural processes such as respiratory transmission and plant water exchange.

Water Droplets - Related Videos

Research

JoVE Journal - Environment

Methane Hydrate Crystallization on Sessile Water Droplets

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Cited by 1 •

2021

We describe a method to form gas hydrate on sessile water droplets to study the effects of various inhibitors, promoters, and substrates on the hydrate crystal morphology.

Title Cell Encapsulation by Droplets

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Cited by 4 •

2007

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

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Cited by 14 •

2016

This manuscript describes a duplex digital PCR assay that can be used to simultaneously quantify Enterococcus spp. and the HF183 genetic markers as indicators of general and human-associated fecal contamination in recreational waters.

Research

JoVE Journal - Biology
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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

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Cited by 19 •

2016

The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles.

Fluorescence detection methods for microfluidic droplet platforms

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Cited by 20 •

2011

Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.

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