Adhesion Force Measurement
Adhesion Force Measurement - Related Videos
09:48
Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
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Cited by 5 •
2015
A protocol to couple a large variety of single molecules covalently onto an AFM tip is presented. Procedures and examples to determine the adhesion force and free energy of these molecules on solid supports and bio-interfaces are provided.
01:14
Adhesion
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2019
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules. Capillary action is a result of water’s adhesive tendencies. When a narrow glass...
09:11
Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
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Cited by 4 •
2015
Here, we present a protocol to continuously quantify cell adhesion and de-adhesion processes with high temporal resolution in a non-invasive manner by cell-substrate impedance and live cell imaging analyses. These approaches reveal the dynamics of cell adhesion/de-adhesion processes triggered by matrix modification and their temporal relationship to adhesion-dependent signaling events.
10:35
Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy
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Cited by 26 •
2016
We present detailed protocols for isolation of aortas from mouse and measurement of their elastic modulus using atomic force microscopy.
11:30
Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers
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Cited by 49 •
2015
Analysis of the contractile properties of chemically skinned, or permeabilized, skeletal muscle fibers offers a powerful means by which to assess muscle function at the level of the single muscle cell. In this article we outline a valid and reliable technique to prepare and test permeabilized skeletal muscle fibers in vitro.
FAQs
The contact portion shows when the probe interacts with the surface, while the pull-off region records the force required as the probe is retracted. The point of detachment marks the end of that interaction. Examining these features together helps researchers interpret bonding or attraction at the interface and compare how strongly different surface conditions resist separation.
Measured adhesion can change when the surface chemistry, solvent, coating, particles, or environmental conditions change. Comparing force curves under different conditions can reveal which chemical or material variables are associated with stronger or weaker attraction. This approach connects an observed mechanical response with the chemistry of the interface and helps identify conditions that modify interfacial behavior.
The measurement is valuable because it converts an interfacial interaction into quantitative force data rather than a purely descriptive observation. At molecular and nanoscale interfaces, those data help clarify how surfaces attract or bond and how that behavior changes across materials or conditions. This makes the technique useful for connecting chemistry at an interface with measurable detachment behavior.
A typical workflow positions the probe relative to the surface, brings the two into contact, and then retracts the probe. During this cycle, the experiment records cantilever deflection or displacement. The recorded response is converted into a force curve, which can then be examined for contact, pull-off, and detachment features that characterize the interaction.
The probe provides the contacting element, the surface supplies the interface being tested, and the cantilever responds through deflection during the contact and retraction cycle. Displacement describes the probe's movement, while cantilever deflection supplies a measurable response that is converted into force. Together, these elements generate the force curve used to analyze adhesion behavior.
In chemistry and materials research, measured adhesion helps compare interfaces relevant to catalysts, coatings, adhesives, sensors, and functional materials. Researchers can examine how surface chemistry, solvents, particles, or environmental conditions influence attraction and detachment. The resulting comparisons support material design while providing insight into the interfacial interactions responsible for different adhesion responses.