Biochip Slide
Biochip Slide - Related Videos
11:19
Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity
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Cited by 12 •
2016
This manuscript describes how to prepare fluidic biochips with Rainbow trout gill epithelial cells for use in a field portable electric cell-substrate impedance sensor. The protocol for running a rapid drinking water toxicity test with the sensor is also described.
01:18
Design Example: Designing Water Slide
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2025
When designing a water slide, controlling the speed of water flow is crucial for rider safety while maintaining an exciting experience. As water flows down the slide, gravity causes it to accelerate, with its speed at the bottom depending on the height from which it starts. The higher the slide, the more potential energy the water has at the top, which is converted into kinetic energy as it descends, increasing its speed. Bernoulli's principle determines the water's velocity along the slide.
06:14
In vitro Biofilm Formation in an 8-well Chamber Slide
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Cited by 62 •
2011
This article describes the procedure for the formation and visualization of a bacterial biofilm grown within an 8-well chamber...
08:03
Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research
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Cited by 3 •
2013
All-solid-state ion-selective electrodes (ASSISEs) constructed from a conductive polymer (CP) transducer provide several months of functional lifetime in liquid media. Here, we describe the fabrication and calibration process of ASSISEs in a lab-on-a-chip format. The ASSISE is demonstrated to have maintained a near-Nernstian slope profile after prolonged storage in complex biological media.
10:04
Visualizing Soil Microorganisms via the Contact Slide Assay and Microscopy
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Soil comprises the thin layer at the earth’s surface, containing biotic and abiotic factors that contribute to life. The abiotic portion includes inorganic particles ranging in size and shape that determine the soil’s texture. The biotic portion incorporates plant residues, roots, organic matter, and microorganisms. Soil microbe abundance and diversity is expansive, as...
FAQs
Each location on the slide carries a defined biological recognition element, such as an antigen, antibody, nucleic acid, or other capture probe. When a matching target binds, a labeled detection molecule produces a signal at that location. Reading multiple locations together links individual signals to different immune or pathogen-associated molecules.
Spatially distinct probe placement creates an address for each interaction. Antigens, antibodies, nucleic acids, and other capture molecules can therefore be assigned to different slide locations, allowing each signal to be connected with a particular immune marker or pathogen-associated molecule. This organization makes multiplexed profiling interpretable rather than a single undifferentiated measurement.
Signal interpretation supports both detection and comparison. A readout can indicate that a target is present and can provide information about its relative abundance among measured targets. Because many interactions are assessed on one slide, researchers can examine patterns of immune markers and pathogen-associated molecules rather than relying on one isolated measurement.
The workflow begins by arranging selected biological molecules or cells on the solid surface. A sample is then exposed to the immobilized probes so matching targets can bind. Labeled detection molecules generate measurable signals, and the pattern across slide locations is interpreted for immune markers or pathogen-associated molecules. Small sample volumes support efficient multiplexed analysis.
Immunology and infection studies can select antigens or antibodies to examine antigen-antibody interactions, nucleic acids to detect matching pathogen-associated targets, and other probes for immune markers. The slide can therefore be configured around host responses, infectious agents, or both. This flexibility helps align the measured panel with the biological question.
Applications include profiling biomarkers, supporting diagnostic development, and examining mechanisms underlying infection and immunity. A high-throughput layout can accelerate these studies by measuring many relevant interactions in parallel while conserving sample volume. The resulting signal pattern provides a broad view of immune or pathogen-associated measurements within one analytical format.