Duodenoscopy Technique

Duodenoscopy is an endoscopic technique that uses a flexible, side-viewing duodenoscope to visualize the duodenum and nearby biliary and pancreatic openings, supporting diagnosis and treatment of gastrointestinal disease. The instrument is advanced through the mouth into the duodenum, where its camera, working channel, and distal elevator guide accessories toward the major papilla; during endoscopic retrograde cholangiopancreatography, contrast can be injected into the bile or pancreatic ducts for imaging. Clinicians use duodenoscopy to evaluate obstruction, bleeding, tumors, and ductal abnormalities and to perform interventions such as stone extraction, biopsy, stent placement, and tissue sampling.

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Education

JoVE Lab Manual - Chemistry

Lab Techniques - Concepts

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2020

Measurement Accuracy The ability to repeat an experiment and get the same results, or reproducibility, is essential in scientific research. However, it’s impossible to repeat an experiment if you don’t know how you did it. Thus, scientists keep detailed records of their experiments in lab notebooks. These records include important information like the amount of each material used and descriptions of each step of the procedure. Equipment, like balances and volumetric or graduated glassware, is...

Lab Techniques - Instructor Prep

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2020

Source: Smaa Koraym at Johns Hopkins University, MD, USA Preparation of LaboratoryExpand Here we show the laboratory preparation for 10 students working individually, with some excess. Please adjust quantities as needed. To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash goggles, and gloves. Obtain enough small plastic bottles with caps for the number of students and label them ‘NaCl’. Use a...

Lab Techniques - Student Protocol

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2020

Source: Smaa Koraym at Johns Hopkins University, MD, USA Determining the Density of an EggExpand Density is a measure of how compact a substance or object is, and it is calculated as mass divided by volume. When a dense object is placed in a liquid with a lower density, the object sinks; when the object is less dense than the liquid, it floats. When the densities are the same, the object will be suspended in the liquid. In this experiment, we'll determine the density of an egg by creating a...

Zebrafish Microinjection Techniques

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2023

One of the major advantages to working with zebrafish (Danio rerio) is that their genetics can be easily manipulated by microinjection of early stage embryos. Using this technique, solutions containing genetic material or knockdown constructs are delivered into the blastomeres: the embryonic cells sitting atop the yolk of the newly fertilized egg. Delivery into the cytoplasm is achieved either through direct injection into the blastomere, or via natural cytoplasmic movements that occur after a...

Rodent Handling and Restraint Techniques

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2015

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN It has been demonstrated that even minimal handling of mice and rats is stressful to the animals. Handling for cage changing and other noninvasive procedures causes an increase in heart rate, blood pressure, and other physiological parameters, such as serum corticosterone levels. Fluctuations can continue for up to several hours. The methods of restraint required for injections and blood...

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