Serial Blood Sampling
Serial Blood Sampling - Related Videos
08:28
Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug
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Cited by 2 •
2021
Detailed microsurgical techniques are demonstrated to establish a longer-term jugular vein cannulation rat model for sequential blood collection in the same animal. Physiological and hematological parameters have been monitored during the rat's recovery phase. This model has been applied to study pharmacokinetics of orally administered polyphenol without inducing animal stress.
07:11
Sampling Blood from the Lateral Tail Vein of the Rat
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Cited by 82 •
2015
Blood samples are useful for assessing biomarkers of physiological states or disease in vivo. Here we describe the methodology to sample blood from the lateral tail vein in the rat. This method provides rapid samples with minimal pain and invasiveness.
10:53
Serial Dilutions and Plating: Microbial Enumeration
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2023
Source: Jonathan F. Blaize1, Elizabeth Suter1, and Christopher P. Corbo1 1 Department of Biological Sciences, Wagner College, 1 Campus Road, Staten Island NY, 10301 Quantitative assessment of prokaryotes can be onerous given their abundance, propensity for exponential proliferation, species diversity within a population, and specific physiological needs. Compounding this challenge, is the four-phase nature in which bacteria replicate (lag, log, stationary and death). The ability to accurately...
01:03
Serial Position Effect
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2025
The serial position effect is a cognitive phenomenon where individuals are more likely to recall the first and last items in a list compared to those in the middle. This effect is divided into the primacy effect and the recency effect. The primacy effect is observed when the initial items in a list are remembered better. This occurs because these items are rehearsed more frequently or receive more elaborative processing, allowing them to be encoded into long-term memory more effectively. For...
09:21
Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples
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Cited by 32 •
2021
This protocol outlines a routine method for using serial block-face scanning electron microscopy (SBF-SEM), a powerful 3D imaging technique. Successful application of SBF-SEM hinges on proper fixation and tissue staining techniques, as well as careful consideration of imaging settings. This protocol contains practical considerations for the entirety of this process.
FAQs
Its value comes from aligning each specimen with a defined biological or clinical time point. Repeated measurements can then be compared with an intervention, treatment event, or disease stage rather than treated as isolated results. This temporal alignment helps reveal changing physiology and supports interpretation of treatment response, drug-related measurements, and progression over the study period.
Reliable trends depend on more than collecting enough specimens. Timing, blood volume, processing, and storage need to be standardized across sampling points, because differences in handling can affect comparability. Controlling these factors produces a consistent series of measurements and also helps limit cumulative blood loss, an important consideration when the same subject contributes multiple samples.
Repeated venipuncture and an indwelling catheter are the two collection approaches identified for this process. The choice affects how specimens are obtained across scheduled time points, while the broader requirement remains consistent timing, volume, processing, and storage. Keeping those conditions controlled allows measurements from separate collections to be interpreted as a longitudinal dataset rather than unrelated samples.
A practical workflow begins by planning the sampling schedule, then collecting each specimen at its assigned time point and recording the relevant intervention or clinical event. The samples must be handled with standardized volume, processing, and storage conditions. This sequence preserves the link between laboratory measurements and the timeline needed to evaluate biological change.
In pharmacokinetic studies, serial blood sampling connects drug-related measurements with the times at which specimens were obtained. The resulting time-linked data can characterize how measurements change after an intervention, rather than providing only one snapshot. That information supports evaluation of dosing and can contribute to evidence used in therapeutic drug monitoring, where measured levels are considered over time.
Medicine uses this approach to follow biomarkers, disease progression, and treatment response. Comparing specimens from the same subject over scheduled points can show whether a measured biological feature changes alongside a clinical event or intervention. The resulting longitudinal evidence may strengthen assessment of diagnosis, dosing, and patient care, while standardized collection makes those comparisons more dependable.