Isoflurane Delivery

Isoflurane delivery is the controlled administration of the volatile anesthetic isoflurane to produce and maintain general anesthesia during medical procedures. An anesthesia machine vaporizes liquid isoflurane and mixes it with a carrier gas before directing the anesthetic through a breathing circuit; inhaled molecules reach the lungs, enter the bloodstream, and depress central nervous system activity. Clinicians adjust delivered concentration and ventilation while monitoring oxygenation, carbon dioxide, circulation, and anesthetic depth. This approach supports predictable anesthetic management, allows rapid adjustments during surgery, and illustrates the importance of precise vaporizer function and respiratory monitoring in perioperative medicine.

Isoflurane Delivery - Related Videos

Research

JoVE Journal - Bioengineering

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

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Cited by 18 •

2016

The goal of this protocol is to use cationic/anionic liposomes with a neuro-targeting peptide as a CNS delivery system to enable siRNA to cross the BBB. The optimization of a delivery system for treatments, like siRNA, would allow for more treatment options for prion and other neurodegenerative diseases.

Targeted Drug Delivery in a Mouse Model via Cisterna Magna Injection

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2025

Source: Zamboni, M., et al., Induction of Leptomeningeal Cells Modification Via Intracisternal Injection. J. Vis. Exp. (2020)This video demonstrates the procedure of delivering an inducer drug into the cisterna magna of an anesthetized mouse. The injection selectively induces fluorescent marker expression in brain surface cells accessible via cerebrospinal fluid, confirmed by fluorescence imaging.

Subpial Gene Delivery in the Mouse Spinal Cord to Evaluate Gene Expression

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2025

Source: Tadokoro, T., et al. Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice. J. Vis. Exp. (2017)This video demonstrates a subpial injection technique for gene delivery to the spinal cord in mice. The procedure involves exposing the dura mater, puncturing the pia mater, and injecting a recombinant adeno-associated virus (rAAV) carrying a fluorescent protein-expressing gene into the subpial space. Following injection and recovery, the virus facilitates gene expression in...

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation

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Cited by 12 •

2010

This protocol describes a non-viral method of delivery of genetic constructs to a certain area of living rodent brain. The method consists of plasmid preparation, micropipette fabrication, neonatal rat pup surgery, microinjection of the construct, and in vivo electroporation.

Intraductal Delivery to the Rabbit Mammary Gland

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Cited by 9 •

2017

Here, we describe a technique for the localized delivery of reagents to the rabbit mammary gland via an intraductal injection. In addition, we describe a protocol for visualization and the confirmation of delivery by high-resolution ultrasound imaging of contrast agents.

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