Perivascular Tissue Excision
Perivascular Tissue Excision - Related Videos
08:15
Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
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Cited by 7 •
2016
Human cardiac tissue harbours multipotent perivascular precursor cell populations that may be suitable for myocardial regeneration. The technique described here allows for the simultaneous isolation and purification of two multipotent stromal cell populations associated with native blood vessels, i.e. CD146+CD34- pericytes and CD34+CD146- adventitial cells, from the human myocardium.
08:28
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
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Cited by 5 •
2017
Here we report a method for isolation of Adipocyte Progenitor Cell (APC) populations from Perivascular Adipose Tissue (PVAT) using Magnetic-activated Cell Sorting (MCS). This method allows for an increased isolation of APC per gram of adipose tissue when compared to Fluorescence-Activated Cell Sorting (FACS).
07:05
Use of Human Perivascular Stem Cells for Bone Regeneration
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Cited by 42 •
2012
Human perivascular stem cells (PSCs) are a novel stem cell class for skeletal tissue regeneration similar to mesenchymal stem cells (MSCs). PSCs can be isolated by FACS (fluorescence activated cell sorting) from adipose tissue procured during standard liposuction procedures, then combined with an osteoinductive scaffold to achieve bone formation in vivo.
09:05
A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
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Cited by 4 •
2017
Here we present a novel clinical grade isolation and culture method for kidney Perivascular Stromal Cells (kPSCs) based on whole organ perfusion with digestive enzymes and NG2-cell enrichment. With this method, it is possible to acquire sufficient cell numbers for cellular therapy.
14:58
A Simplified Technique for In situ Excision of Cornea and Evisceration of Retinal Tissue from Human Ocular Globe
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Cited by 7 •
2012
The paper describes a simplified technique to excise corneal and to eviscerate retinal tissues from the ocular globe of human cadaveric donors. The technique described here will help to excise good quality tissues to be used for transplantation, surgical or research purposes without damaging other tissues of the ocular globe.
FAQs
The surgeon follows tissue planes next to the vessel to separate the target tissue while limiting unnecessary disruption. This controlled dissection helps identify the boundary between the specimen and the vascular wall, supporting removal of the intended tissue without compromising vessel integrity. The quality of this separation directly affects exposure, specimen retrieval, and preservation of blood flow.
Because the operative field lies immediately around an artery or vein, uncontrolled bleeding can interfere with dissection and obscure the relationship between the tissue and vessel. Careful bleeding control maintains visibility, while preserving the vessel protects ongoing blood flow. These priorities allow the surgeon to obtain the target tissue or access without creating avoidable vascular damage.
The removed tissue can be submitted for histopathological analysis, in which its structure and cellular features are examined to support clinical interpretation. This is particularly relevant when tissue near the vascular wall may represent a tumor or an inflammatory abnormality. The specimen therefore provides diagnostic information in addition to its immediate surgical purpose.
The procedure begins with careful dissection through tissue planes adjacent to the blood vessel. The surgeon then separates the target tissue from the vessel, controls bleeding during the process, and protects the vessel so its integrity and blood flow remain intact. After removal, the specimen may be directed for histopathological analysis, depending on the clinical objective.
Its applications include vascular surgery, assessment of tumors near a vessel, management of inflammatory conditions, and procedures requiring precise exposure of arteries or veins. The same technique can therefore serve different goals: obtaining tissue for diagnosis, improving access to the vascular region, or addressing a localized problem affecting the space around the vessel.
Excision may create clearer access to an artery or vein by removing tissue that obstructs the operative field. In selected situations, removing localized tissue can also relieve compression near the vessel. The resulting benefit depends on maintaining vascular integrity and blood flow while achieving the intended exposure or decompression, rather than simply removing the greatest possible amount of tissue.