Rat Model Surgery
Rat Model Surgery - Related Videos
09:39
Creation of Reversible Cholestatic Rat Model
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2011
Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Creation of a reversible cholestatic rat model can be challenging in view of the smaller size and unique hepatopancreatobiliary anatomy in rats. This video article demonstrates the creation of a reversible cholestatic model.
07:23
Surgery and Behavioral Testing in the Tibial Neuroma Transposition Model in Rats
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Cited by 2 •
2023
This protocol describes the tibial neuroma transposition model, which entails a lesion of the tibial nerve with subsequent transposition of the proximal nerve end toward a subcutaneous pretibial or lateral position. Behavioral testing of neuroma pain and plantar hyperalgesia is quantified using Von Frey monofilaments.
09:23
Shunt Surgery, Right Heart Catheterization, and Vascular Morphometry in a Rat Model for Flow-induced Pulmonary Arterial Hypertension
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Cited by 15 •
2017
This protocol describes a surgical procedure to create a model for flow-induced pulmonary arterial hypertension (PAH) in rats and the procedures to analyze the principle hemodynamic and histological end-points in this model.
12:45
Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat
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Cited by 22 •
2012
Targeted ablation of specific brain region(s) by infusion of an excitotoxin using stereotaxic coordinates is described. This technique could also be adapted for infusion of other chemicals into the rat brain.
00:05
Facial Nerve Surgery in the Rat Model to Study Axonal Inhibition and Regeneration
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Cited by 12 •
2020
This protocol describes a reproducible approach to facial nerve surgery in the rat model, including descriptions of various inducible patterns of injury.
FAQs
These safeguards address different sources of experimental risk. Anesthesia supports humane surgery, aseptic conditions help limit complications associated with contamination, and vital-sign monitoring provides information about the animal’s status during the procedure. Together, they help researchers protect animal welfare while improving confidence that observed findings reflect the intended intervention rather than avoidable surgical problems.
The selected intervention depends on the scientific question being studied. A defined injury can support disease or repair research, whereas an implanted device can be used to evaluate a device or biomaterial. Altering an organ or vessel addresses specific physiological systems, while tissue collection provides material for examining anatomy, disease mechanisms, or treatment-related changes.
Standardized procedures make surgical interventions more consistent across animals and experiments. Greater consistency can reduce variation caused by differences in how the operation is performed, helping researchers compare outcomes more reliably. This is particularly important when evaluating drugs, surgical techniques, biomaterials, or regenerative therapies, because reproducible methods strengthen interpretation of treatment-related findings.
Its applications span several major areas of medicine, including neurological, cardiovascular, orthopedic, and metabolic disorders. The same general surgical framework can therefore be adapted to study different organs, tissues, and disease mechanisms. This breadth also allows researchers to investigate both biological processes and potential interventions within controlled experimental settings.
A typical study begins with anesthesia and preparation under aseptic conditions, followed by the planned injury, implantation, organ or vessel alteration, or tissue collection. Researchers monitor vital signs during the operation, then provide postoperative care. This sequence connects the experimental intervention with immediate safety measures and subsequent recovery management.
Researchers can use these models to examine surgical techniques, biomaterials, drugs, and regenerative therapies in relation to defined anatomical or physiological problems. Depending on the design, the procedure may create a relevant injury, introduce a device, or alter a target structure before outcomes are assessed. This supports controlled investigation of treatment effects and repair-related responses.
Ethical oversight helps ensure that the scientific purpose justifies the animal use and that procedures are planned responsibly. Postoperative care addresses the animal’s condition after surgery, while monitoring and standardized methods help limit pain, complications, and unnecessary variation. These requirements support both humane treatment and the production of scientifically interpretable results.