Wide-area Imaging
Wide-area Imaging - Related Videos
10:05
A Large Lateral Craniotomy Procedure for Mesoscale Wide-field Optical Imaging of Brain Activity
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Cited by 18 •
2017
This protocol presents a method for creating a large unilateral craniotomy over the temporal and parietal regions of the mouse cerebral cortex. This is especially useful for real time imaging over an expansive area of a cortical hemisphere.
06:42
Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
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Cited by 32 •
2013
We review our recent results on the integration of fluorescent microscopy and imaging flow cytometry tools on a cell-phone using compact and cost-effective opto-fluidic attachments. These cell-phone based micro-analysis devices might be useful for cytometric analysis, such as performing various cell counting tasks as well as for high-throughput screening of e.g., water samples in resource limited settings.
06:45
In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window
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Cited by 9 •
2022
The present protocol describes making a large (6 x 3 mm2) cranial window using food wrap, transparent silicone, and cover glass. This cranial window allows in vivo wide-field and two-photon calcium imaging experiments in the same mouse.
06:50
Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis
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Cited by 7 •
2021
Extracellular glutamate-triggered systemic calcium signaling is critical for the induction of plant defense responses to mechanical wounding and herbivore attack in plants. This article describes a method to visualize the spatial and temporal dynamics of both these factors using Arabidopsis thaliana plants expressing calcium- and glutamate-sensitive fluorescent biosensors.
FAQs
Optics direct signals from an expanded anatomical region toward detectors, which record the available information across the broader field. Image-processing methods then organize or assemble those signals into a coherent image while retaining clinically useful contrast and structural detail. This coordination allows the system to represent spatial relationships across a region rather than isolating each feature in a narrowly focused view.
Image assembly converts information collected during a coordinated series into a detailed representation of the larger region. The processing step helps preserve continuity between areas and supports interpretation of spatial patterns across the field. In medical assessment, that broader continuity can reveal relationships among tissue features, lesions, or vessels that may be difficult to appreciate when viewed only as separate smaller scans.
The approach combines signal recording with image processing designed to retain contrast and structural information while covering more anatomy. Preserving these features matters because a broad image must remain interpretable, not merely extensive. Clinicians can therefore evaluate tissue appearance and spatial organization across the captured region, supporting assessment of patterns that depend on both local detail and wider context.
Its main added value is spatial context. A smaller scan may show local anatomy clearly but fail to display how findings are distributed across a larger region. By covering more area in one acquisition or a coordinated series, Wide-area Imaging can help reveal broader lesion or vascular patterns and support interpretation of anatomical relationships that a limited field may miss.
The workflow begins by capturing anatomical or physiological signals across the intended broad region, either in one acquisition or through a coordinated series. Detectors record the signals, and image-processing methods organize them into a detailed image. The resulting view can then be examined for tissue characteristics, structural relationships, lesions, or vascular patterns relevant to the clinical question.
Clinicians may use it when the distribution of tissue findings or lesions across a broad region is important to evaluation. The expanded view supports assessment of spatial patterns rather than only isolated abnormalities. This can contribute to diagnosis and treatment planning by showing where findings occur in relation to surrounding anatomy and to one another.
For surgical guidance, the broader image can provide anatomical or lesion context during planning or intervention. In screening, it can help survey an expanded region for relevant patterns. During longitudinal monitoring, repeated broad views can support comparison of findings across examinations. These uses extend the technique from image capture to clinical decision-making, treatment planning, and follow-up assessment.