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Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis
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Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

DOI:
10.3791/62114-v

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06:50 min

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June 04, 2021

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, , , ,
1Department of Biochemistry and Molecular Biology,Saitama University, 2Department of Botany,University of Wisconsin

Chapters

  • 00:04Introduction
  • 00:56Microscope Setting and Conducting Real-time Imaging
  • 02:48Data Analysis
  • 04:23Results: Ca2+ Transmission and Elevation of Apoplastic Glutamate in Response to Mechanical Wounding
  • 06:09Conclusion

Summary

Automatic Translation

Automatic Translation

Extracellular glutamate-triggered systemic calcium signaling is critical for the induction of plant defense responses to mechanical wounding and herbivore attack in plants. This article describes a method to visualize the spatial and temporal dynamics of both these factors using Arabidopsis thaliana plants expressing calcium- and glutamate-sensitive fluorescent biosensors.

Tags

Keywords: Real-time ImagingPlant Systemic SignalingCalciumApoplastic GlutamateWound ResponseBiotic And Abiotic StressFluorescence MicroscopyArabidopsis

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