Intradermal Injection Technique
Intradermal Injection Technique - Related Videos
08:46
Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites
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Cited by 12 •
2016
We describe here a protocol for isolating myeloid cells from mouse skin and draining lymph node following intradermal injection of Plasmodium sporozoites. Flow cytometry of collected cells provides a reliable assay to characterize the skin and draining lymph node inflammatory response to the parasite.
07:34
Minimally-invasive Technique for Injection into Rat Optic Nerve
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Cited by 4 •
2015
Direct injection into the rat optic nerve is useful for regenerative research. We demonstrate a minimally-invasive technique for direct injection into a rat optic nerve that does not involve opening the skull. Using this method, surgical complications are minimized and recovery is more rapid.
09:08
Facilitating Repeat Intracarotid Injections in Mouse Models by a Novel Injection Site Repair Technique
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Cited by 1 •
2024
Repair of the intracarotid artery in a mouse model after injection returns blood flow to the artery without negatively impacting the distribution of the injected material. Injection site repair facilitates subsequent injections through the same artery and prevents cerebral ischemia in mouse strains that lack a complete Circle of Willis.
03:15
A Hypertonic Saline Injection Technique for Inducing Glaucoma in Rats
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2025
This video demonstrates glaucoma induction in an anesthetized rat by injecting hypertonic saline into the episcleral vein. The saline enters the trabecular meshwork via Schlemm's canal, causing tissue scarring, which blocks aqueous humor outflow and raises intraocular pressure. The elevated pressure damages retinal ganglion cells, leading to glaucoma.
02:19
Intradermal Immunization with Live Attenuated Sporozoites in a Mouse Model
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2025
This video demonstrates an assay for immunizing a mouse model with live attenuated sporozoites. The radiation-attenuated Plasmodium sporozoites are injected into the dorsal side of the mouse's ear. After injections at multiple locations on both ears, the mouse is allowed to recover.
FAQs
The upward-facing bevel helps the needle enter the dermis at a shallow trajectory, while controlled pressure limits how deeply the material is driven. The resulting bleb serves as an immediate placement cue. This matters because positioning within the intended layer keeps the stimulus close to dermal sensory and immune cells.
Small injected volumes are important because the technique is designed to create a localized exposure rather than broadly distribute material through deeper tissue. A confined stimulus can be related more directly to nearby peripheral nerve endings and local immune responses, helping investigators examine cutaneous signaling with greater experimental control during a controlled experiment.
Compared with delivery into deeper tissue, intradermal placement keeps the test material nearer the skin’s sensory interface. That distinction is useful when the research question concerns pain, itch, inflammation, or cutaneous sensory signaling. It also helps separate local skin responses from effects that could arise after deeper or less spatially restricted delivery.
The dermis provides a site where an introduced stimulus can interact with both local sensory and immune cells. Sensory-cell engagement is relevant to peripheral signaling, while immune-cell involvement helps frame local inflammatory responses. Considering both cell populations allows experiments to connect a skin-level intervention with pain, itch, and inflammation research.
Two observations are central: the needle enters at a shallow angle with the bevel facing upward, and controlled pressure produces a small raised bleb. Together, these cues indicate that the material has been placed in the dermis. Recording them consistently can improve comparability among experimental applications and local response measurements.
Researchers may select it when they need to introduce a defined stimulus near peripheral nerve endings and then evaluate a local sensory response. The approach is especially relevant to pain, itch, inflammation, and cutaneous sensory signaling. Its localized delivery can also support comparisons of sensory processing while limiting the experimental focus to a defined skin site.