Stellate Cells
Stellate Cells - Related Videos
06:40
Seven Steps to Stellate Cells
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Cited by 67 •
2011
Here we describe a method for the isolation of hepatic stellate cells from mouse liver. For stellate cell purification, mouse livers are digested in situ and in vitro by pronase-collagenase treatment prior to density gradient centrifugation. This technique yields highly pure hepatic stellate cells.
07:50
Location, Dissection, and Analysis of the Murine Stellate Ganglion
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Cited by 15 •
2020
Pathophysiological changes in the cardiac autonomic nervous system, especially in its sympathetic branch, contribute to the onset and maintenance of ventricular arrhythmias. In the present protocol, we show how to characterize murine stellate ganglia to improve the understanding of the underlying molecular and cellular processes.
08:08
Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model
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Cited by 16 •
2014
This article provides detailed methodologies for the use of three-dimensional (3D) assays to quantify breast cancer cell invasion. Specifically, we discuss the procedures required to set up such assays, quantification, and data analysis, as well as methods to examine the loss of membrane integrity that occurs when cells invade.
04:34
3D Culture Basement Membrane Assay: Culturing Cancer Cells on 3D Basement Membrane Protein Matrix to Study Cell Invasion
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2023
This video provides a detailed methodology to grow breast cancer cells in a 3D basement membrane protein matrix, exemplifying the potential of 3D culture in the assessment of cell invasion into the surrounding environment.
08:29
Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures
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Cited by 15 •
2008
Here we show our method to isolate mouse epidermal neural crest stem cells (EPI-NCSC). Technique involves micro-dissecting whisker follicles, isolating the bulge and placeing it into tissue culture. EPI-NCSC start to emigrate from bulge explants onto the substratum within 3 - 4 days.
FAQs
Their radiating dendrites provide multiple sites for receiving inputs from different sources, allowing the cell to combine signals before influencing downstream targets. This arrangement makes dendritic organization functionally important rather than merely structural. In neuroscience research, examining how branching patterns relate to synaptic connectivity helps explain how local circuits transform incoming activity into coordinated neural responses.
A stellate cell can either promote or restrain activity, depending on its type and brain region. Excitatory cells strengthen signaling to their targets, whereas inhibitory cells limit or shape circuit activity. Distinguishing these effects is essential when interpreting connectivity, because the same broad cellular morphology can contribute to very different patterns of information flow.
Within the cerebral cortex, stellate cells contribute to the handling of sensory information through their local synaptic relationships and signal integration. Their function is therefore linked to how cortical circuits combine inputs rather than to morphology alone. Studying these neurons can clarify how cellular structure and neurotransmitter release support the transformation of sensory signals within cortical networks.
Cerebellar stellate cells help regulate activity within cerebellar microcircuits, where the balance and timing of local signals are important for circuit operation. Their contribution depends on how they connect with neighboring elements and whether their signaling is excitatory or inhibitory. Investigating these relationships provides a cellular perspective on how cerebellar networks control local neural activity.
A useful analysis considers morphology, synaptic connectivity, and neurotransmitter release together. Morphology reveals the organization of dendrites and axons, connectivity identifies potential sources and targets of signals, and transmitter release indicates how those connections affect circuit activity. Combining these features gives a more complete account of stellate-cell function than examining shape or location in isolation.
Studies of these neurons can address how local circuits function, how neural connections develop, and how circuit activity may change in neurological disease. Researchers can relate cellular structure to connectivity and signaling to determine how alterations might affect information processing. This makes stellate cells relevant both to basic neuroscience and to investigations of disrupted neural circuitry.