Name: 高塩食条件下での塩分感受性ラットにおける腸内微生物細胞外小胞の分離と鑑別特性評価 (動画) | JoVE Journal
Uploaded: 2025-06-06T15:45:00.000+00:00
Duration: 7 min 21 s
Description: 学ぶ 密度勾配遠心法による高塩食ラットの腸内微生物細胞外小胞の分離と特性評価。ナノ粒子追跡分析と電子顕微鏡でサイズ・形態を解析し、高血圧研究における腸内微生物叢の役割を解明する方法です。

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高塩食条件下での塩分感受性ラットにおける腸内微生物細胞外小胞の分離と鑑別特性評価

Separation and Differential Characterization of Gut Microbial Extracellular Vesicles in Salt-Sensitive Rats under High-Salt Diet Conditions

EVの濃度は、異なる分画で測定しました(図2A)。実験結果は、EVの濃度が一連の密度勾配溶液で典型的な正規分布パターンを示すことを示しました(図2B)。具体的には、第9分数では、EVの濃度が最高点(3.85×109)に達しており、EVの主分布分が936である可能性が示唆されている。
タンパク質含有量の測定には、BCAキットを使用して、さまざまな画分中のタンパク質含有量を評価しました(図2C)。この方法では、フラクション9のタンパク質含有量は0.417μg/μLであり、EVの分布をさらに示しています。LPSはグラム陰性菌37,38のユニークな成分であるため、エンドトキシン検出キットもLPSの発現を決定するために使用され(<...

High salt intake is a major risk factor for hypertension, and its underlying mechanism may be closely linked to extracellular vesicles (EVs) secreted by ...

separation-differential-characterization-gut-microbial-extracellular

Research

JoVE Journal

Biochemistry

生物化学

出典:Cian、MB et al.、アシネトバクターバウマンニの技術的調整によるグラム陰性菌の細胞エンベロープの内膜画分と外膜画分の分離。J. Vis.

グラム陰性菌の内膜画分と外膜画分の分離

出典: ワトソン、DC、 et.al。大腸菌と他の細菌からの細胞外小胞のスケーラブルな単離と精製 J. Vis. 経験。 (2021年)このビデオは、遺伝子組み換え細菌の外膜に由来する細胞外小胞(EV)の単離と濃縮を示しています。このプロセスには、細菌を培養して EV...

細菌からの外膜由来細胞外小胞の単離と濃縮

結核菌は、低鉄条件に応答して細胞外小胞の産生および放出の増加を示す。本研究では、鉄欠乏症に反応して放出されるマイコバクテリア細胞外小胞の精製および特性評価のための低鉄条件および特性評価のための方法を生成するためのプロトコルを詳しく説明する。...

鉄制限性抗酸菌による細胞外小胞の単離と特徴付け

細菌性小胞は病因において重要な役割を果たし、有望なバイオテクノロジーの応用を有する。小胞の不均一性は分析と使用を複雑にします。したがって、小胞の様々なサイズを分離する簡単で再現可能な方法が必要です。ここでは、...

細菌外膜小胞ヘテロジネミティーを分析するサイズ排除クロマトグラフィー

細菌は、生理活性のある生体分子を運ぶナノメートルサイズの細胞外小胞(EV)を分泌します。EVの研究は、それらの生合成、微生物-微生物および宿主-微生物の相互作用および疾患における役割、ならびにそれらの潜在的な治療用途を理解することに焦点を当てています。EV研究の標準化を促進するために、さまざまな細菌からEVをスケーラブルに分離するためのワークフローが提示されます。...

大腸菌およびその他の細菌からの細胞外小胞のスケーラブルな単離および精製(英語)

本プロトコールは、シアノバクテリア培養物からの細胞外小胞の単離、濃縮、および特性評価に関する詳細な説明を提供する。異なるスケールの培養物から小胞を精製するためのアプローチ、方法論間のトレードオフ、およびフィールドサンプルを扱うための考慮事項についても議論する。...

シアノバクテリア細胞外小胞の単離と特性評価

ここでの目標は、心血管疾患における嚥下障害のメカニズムを調査するためのプロトコルの概要を説明することです。本稿では、マウスの糞便を無菌的に採取・移植し、腸管を分離し、「スイスロール」法を用いて消化管の変化を調べる免疫染色法について論じる。...

マウス糞便分離と微生物叢移植

本研究では、密度勾配遠心分離(DGC) により...

密度勾配遠心分離法を用いたヒト糞便からの細菌細胞外小胞の単離と精製(英語)

本記事は以下の通りです:(1) DOL 6の時点で新生マウスにテスト分子を経口で投与すること;および(2) DOL 10から離乳(DOL 20)までの生検における大腸上皮障壁機能の生検におけるエクス ビビ...

新生マウスの子マウスにおける口腔ガベージと、使用チャンバーを用いた腸のバリア健全性の機能評価

腸宿主微生物間相互作用は、合成経口コミュニティ、体外消化消化、および小腸上皮のモデルを組み合わせる手法を使用して評価されました。病原体と複数種バイオ...

培養腸宿主微生物インターフェイスで合成細菌コンソーシアムの実行可能性を評価します。

学ぶ 密度勾配遠心法による高塩食ラットの腸内微生物細胞外小胞の分離と特性評価。ナノ粒子追跡分析と電子顕微鏡でサイズ・形態を解析し、高血圧研究における腸内微生物叢の役割を解明する方法です。

高塩食条件下での塩分感受性ラットにおける腸内微生物細胞外小胞の分離と鑑別特性評価

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