Monitoring Epileptiform Events in Drosophila via Calcium Imaging

October 13th, 2023

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Ming-Feng He¹, Chu-Qiao Liu², Xi-Xing Zhang², Yong-Miao Lin², Yu-Ling Mao^3,4, Jing-Da Qiao¹

¹Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, the Second Affiliated Hospital, Guangzhou Medical University, ²The Second Clinical Medicine School of Guangzhou Medical University, ³Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, ⁴Key Laboratory for Reproductive Medicine of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University

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כאן, אנו מציגים פרוטוקול להדמיית סידן ex vivo בדרוזופילה למבוגרים המבטאים GCaMP6 לניטור פעילויות אפילפטיפורמיות. הפרוטוקול מספק כלי רב ערך לחקר אירועים איקטליים בדרוזופילה בוגרת באמצעות הדמיית סידן ex vivo , המאפשר לחקור את המנגנונים הפוטנציאליים של אפילפסיה ברמה התאית.

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Monitoring Epileptiform Events in Drosophila via Calcium Imaging

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