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JoVE Science Education

Advanced Biology

Visual demonstrations of key scientific experiments

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6 Chapters

85 Videos

400+ Multiple Choice Questions

Chapter 1

Neuroscience

15 Videos · 134 Minutes

An Introduction to Neurophysiology

1.1: An Introduction to Neurophysiology

95.3K views

Patch Clamp Electrophysiology

1.2: Patch Clamp Electrophysiology

95.7K views

Calcium Imaging in Neurons

1.3: Calcium Imaging in Neurons

95.2K views

An Introduction to Neuroanatomy

1.4: An Introduction to Neuroanatomy

62.5K views

Rodent Stereotaxic Surgery

1.5: Rodent Stereotaxic Surgery

150K views

Histological Staining of Neural Tissue

1.6: Histological Staining of Neural Tissue

157.3K views

An Introduction to Behavioral Neuroscience

1.7: An Introduction to Behavioral Neuroscience

46.4K views

The Morris Water Maze

1.8: The Morris Water Maze

52.2K views

fMRI: Functional Magnetic Resonance Imaging

1.9: fMRI: Functional Magnetic Resonance Imaging

58.2K views

An Introduction to Cellular and Molecular Neuroscience

1.10: An Introduction to Cellular and Molecular Neuroscience

72.2K views

Primary Neuronal Cultures

1.11: Primary Neuronal Cultures

67.6K views

Neuronal Transfection Methods

1.12: Neuronal Transfection Methods

16K views

Explant Culture of Neural Tissue

1.13: Explant Culture of Neural Tissue

23.2K views

Murine <i>In Utero</i> Electroporation

1.14: Murine In Utero Electroporation

21K views

An Introduction to Developmental Neurobiology

1.15: An Introduction to Developmental Neurobiology

39.6K views

Chapter 2

Developmental Biology

15 Videos · 131 Minutes

An Introduction to Developmental Genetics

2.1: An Introduction to Developmental Genetics

37.2K views

Gene Silencing with Morpholinos

2.2: Gene Silencing with Morpholinos

34.7K views

Genetic Engineering of Model Organisms

2.3: Genetic Engineering of Model Organisms

21.5K views

An Introduction to Molecular Developmental Biology

2.4: An Introduction to Molecular Developmental Biology

32.1K views

Explant Culture for Developmental Studies

2.5: Explant Culture for Developmental Studies

24.3K views

Whole-Mount <em>In Situ</em> Hybridization

2.6: Whole-Mount In Situ Hybridization

65.8K views

An Introduction to Stem Cell Biology

2.7: An Introduction to Stem Cell Biology

37K views

Embryonic Stem Cell Culture and Differentiation

2.8: Embryonic Stem Cell Culture and Differentiation

35.7K views

Induced Pluripotency

2.9: Induced Pluripotency

26.8K views

An Introduction to Organogenesis

2.10: An Introduction to Organogenesis

35.5K views

2.11: Fate Mapping

61.4K views

Transplantation Studies

2.12: Transplantation Studies

8.7K views

An Introduction to Aging and Regeneration

2.13: An Introduction to Aging and Regeneration

14.4K views

Invertebrate Lifespan Quantification

2.14: Invertebrate Lifespan Quantification

6.2K views

Tissue Regeneration with Somatic Stem Cells

2.15: Tissue Regeneration with Somatic Stem Cells

21K views

Chapter 3

Genetics

16 Videos · 150 Minutes

An Overview of Genetic Analysis

3.1: An Overview of Genetic Analysis

42.5K views

Genetic Crosses

3.2: Genetic Crosses

61.8K views

Genetic Screens

3.3: Genetic Screens

31K views

An Overview of Genetics and Disease

3.4: An Overview of Genetics and Disease

54.4K views

3.5: SNP Genotyping

75.3K views

3.6: Cytogenetics

44.7K views

An Overview of Gene Expression

3.7: An Overview of Gene Expression

78.5K views

Expression Profiling with Microarrays

3.8: Expression Profiling with Microarrays

35K views

Rapid Amplification of cDNA Ends

3.10: Rapid Amplification of cDNA Ends

15.5K views

3.11: RNA-Seq

72.5K views

An Overview of Epigenetics

3.12: An Overview of Epigenetics

50.8K views

DNA Methylation Analysis

3.13: DNA Methylation Analysis

27.6K views

Chromatin Immunoprecipitation

3.14: Chromatin Immunoprecipitation

48.9K views

An Overview of Genetic Engineering

3.15: An Overview of Genetic Engineering

41K views

Recombineering and Gene Targeting

3.16: Recombineering and Gene Targeting

29.7K views

3.17: Genome Editing

54.8K views

Chapter 4

Cell Biology

15 Videos · 137 Minutes

An Introduction to Cell Division

4.1: An Introduction to Cell Division

78.9K views

Cell Cycle Analysis

4.2: Cell Cycle Analysis

60.8K views

Live Cell Imaging of Mitosis

4.3: Live Cell Imaging of Mitosis

77.8K views

An Introduction to Cell Motility and Migration

4.4: An Introduction to Cell Motility and Migration

34.9K views

The Transwell Migration Assay

4.5: The Transwell Migration Assay

127.6K views

Invasion Assay Using 3D Matrices

4.6: Invasion Assay Using 3D Matrices

13.3K views

An Introduction to Endocytosis and Exocytosis

4.7: An Introduction to Endocytosis and Exocytosis

78K views

Cell-surface Biotinylation Assay

4.8: Cell-surface Biotinylation Assay

79.5K views

FM Dyes in Vesicle Recycling

4.9: FM Dyes in Vesicle Recycling

13K views

An Introduction to Cell Metabolism

4.10: An Introduction to Cell Metabolism

44K views

The ATP Bioluminescence Assay

4.11: The ATP Bioluminescence Assay

47.8K views

Detecting Reactive Oxygen Species

4.12: Detecting Reactive Oxygen Species

32.6K views

An Introduction to Cell Death

4.13: An Introduction to Cell Death

55.4K views

The TUNEL Assay

4.14: The TUNEL Assay

92.5K views

Annexin V and Propidium Iodide Labeling

4.15: Annexin V and Propidium Iodide Labeling

75.8K views

Chapter 5

Immunology

12 Videos · 152 Minutes

Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes

5.1: Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes

97.8K views

Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes

5.2: Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes

25.1K views

ELISA Assays: Indirect, Sandwich, and Competitive

5.3: ELISA Assays: Indirect, Sandwich, and Competitive

245.4K views

ELISPOT Assay: Detection of IFN-γ Secreting Splenocytes

5.4: ELISPOT Assay: Detection of IFN-γ Secreting Splenocytes

29.5K views

Immunohistochemistry and Immunocytochemistry: Tissue Imaging via Light Microscopy

5.5: Immunohistochemistry and Immunocytochemistry: Tissue Imaging via Light Microscopy

81.2K views

Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas

5.6: Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas

44.9K views

Immunofluorescence Microscopy: Immunofluorescence Staining of Paraffin-Embedded Tissue Sections

5.7: Immunofluorescence Microscopy: Immunofluorescence Staining of Paraffin-Embedded Tissue Sections

55.3K views

Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts

5.8: Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts

44.5K views

Immunoprecipitation-Based Techniques: Purification of Endogenous Proteins Using Agarose Beads

5.9: Immunoprecipitation-Based Techniques: Purification of Endogenous Proteins Using Agarose Beads

89.5K views

Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry

5.10: Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry

25.4K views

Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS

5.11: Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS

23.4K views

Assay for Cell Death: Chromium Release Assay of Cytotoxic Ability

5.12: Assay for Cell Death: Chromium Release Assay of Cytotoxic Ability

151.9K views

Chapter 6

Microbiology

12 Videos · 134 Minutes

Creating a Winogradsky Column: A Method to Enrich the Microbial Species in a Sediment Sample

6.1: Creating a Winogradsky Column: A Method to Enrich the Microbial Species in a Sediment Sample

133.8K views

Serial Dilutions and Plating: Microbial Enumeration

6.2: Serial Dilutions and Plating: Microbial Enumeration

329.2K views

Enrichment Cultures: Culturing Aerobic and Anaerobic Microbes on Selective and Differential Medias

6.3: Enrichment Cultures: Culturing Aerobic and Anaerobic Microbes on Selective and Differential Medias

136.8K views

Pure Cultures and Streak Plating: Isolation of Single Bacterial Colonies from a Mixed Sample

6.4: Pure Cultures and Streak Plating: Isolation of Single Bacterial Colonies from a Mixed Sample

173.2K views

16S rRNA Sequencing: A PCR-based Technique to Identify Bacterial Species

6.5: 16S rRNA Sequencing: A PCR-based Technique to Identify Bacterial Species

200.3K views

Growth Curves: Generating Growth Curves Using Colony Forming Units and Optical Density Measurements

6.6: Growth Curves: Generating Growth Curves Using Colony Forming Units and Optical Density Measurements

317.2K views

Antibiotic Susceptibility Testing: Epsilometer Tests to Determine MIC Values of Two Antibiotics and Evaluate Antibiotic Synergy

6.7: Antibiotic Susceptibility Testing: Epsilometer Tests to Determine MIC Values of Two Antibiotics and Evaluate Antibiotic Synergy

96.6K views

Microscopy and Staining: Gram, Capsule, and Endospore Staining

6.8: Microscopy and Staining: Gram, Capsule, and Endospore Staining

377.2K views

Plaque Assay: A Method to Determine Viral Titer as Plaque Forming Units (PFU)

6.9: Plaque Assay: A Method to Determine Viral Titer as Plaque Forming Units (PFU)

193.2K views

Transformation of <em>E. coli</em> Cells Using an Adapted Calcium Chloride Procedure

6.10: Transformation of E. coli Cells Using an Adapted Calcium Chloride Procedure

89.7K views

Conjugation: A Method to Transfer Ampicillin Resistance from Donor to Recipient <em>E. coli</em>

6.11: Conjugation: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli

41.2K views

Phage Transduction: A Method to Transfer Ampicillin Resistance from Donor to Recipient <em>E. coli</em>

6.12: Phage Transduction: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli

31.6K views

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Visualized experiments

Step-by-step video demonstrations of key lab experiments and theory behind.