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Encyclopedia of Experiments

Prostate Cancer

This collection features techniques employed in prostate cancer research relating to cancer cell/organoid culture, processing, and staining, cell and tissue sampling, organ extractions, in vivo & in vitro cancer modeling, and cancer proteomics studies.

This collection features research techniques for the model organism Drosophila melanogaster at each stage of its life cycle. These methods are used by Drosophila researchers to explore a wide range of physiological and behavioral questions.

Assays and techniques

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Peptide Purification An RP-HPLC-based Technique to Extract Peptides from Digested Protein Lysates

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Protein Extraction and Proteolysis A Method to Obtain Proteins from Prostate Tumor Tissue Samples and their Enzymatic Digestion into Peptides

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ADI-based Autophagy Induction in Prostate Cancer Cells An Enzyme-based Technique to Measure Autophagic Response in Cells

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Agarose Spin Down Assay A Technique to Embed Organoids for Histological Analysis

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Prostate Organoid Assay A Matrix Gel Ring-based Ex Vivo Culture Technique to Study the Differentiation Capacity of Prostate Epithelial Cells

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Protein Lysate Extraction A Technique to Lyse Organoids to Collect Cellular Proteins

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Whole-mount Immunofluorescence Imaging A Fixing and Staining Technique to Evaluate Intact Organoids

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Sampling Prostate Cancer Tissue for Biobanking An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue

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Functionalized Wire-based Target Cell Isolation An Ex Vivo Technique to Isolate Cancer Cells from Spiked Peripheral Blood

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Single Cell Sampling Using Micromanipulation A Technique to Isolate Single Cells from Cancer Cell Suspension

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Single Cell Sampling Using Laser Capture Microdissection A Technique to Harvest Target Cells from a Heterogeneous Cell Population

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Preparing Organoids from Tumors A 3D In Vitro Tumor Model

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Generating Chemoresistant Prostate Cancer Cells A Procedure for Obtaining Drug-resistant Cancer Cells In Vitro

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Functional Chemoresistance Characterization A Method to Evaluate Drug Resistance in Cancer Cells Using Clonogenic Assay

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Phosphopeptide Enrichment An Antibody-based Immunoprecipitation Technique to Separate Specific Phosphorylated Peptides from Complex Peptide Mixtures

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Titanium Dioxide-based Enrichment of Phosphopeptides A Method for the Selective Isolation of Phosphopeptides from Peptide Library

Animal model studies

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Modeling Prostate Cancer in Genetically-engineered Mouse Models A CRISPR/Cas9-mediated Localized Gene Editing Technique in Mouse Anterior Prostate Lobe Cells

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Intra-prostatic Injection of Cancer Cells A Technique to Deliver Cancer Cells for Establishing Orthotopic Prostate Cancer Mouse Model

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Renal Capsule Tumor Xenografting A Technique to Generate an Experimental Animal Model to Evaluate Prostate Carcinogenesis

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Extracting Prostate from Mouse Urogenital System A Technique to Separate Prostate Gland from Other Urinary and Genital System Organs in Male Mouse

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Individual Lobe Extraction from Prostate A Technique to Harvest Individual Prostate Lobes from Mouse Prostate

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