Zhejiang Provincial Dermatology Hospital 1 article published in JoVE Cancer Research A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells Yuwei Qi*1, Yuhou Liu*1, Ye Huang2, Mengqing Xiong1, Shumei You2, Bo Wang3, Mancang Gu1,3 1School of Pharmaceutical Science of Zhejiang Chinese Medical University, 2Department of Pharmacy, Zhejiang Provincial Dermatology Hospital, 3Academy of Chinese Medical Sciences of Zhejiang Chinese Medical University This protocol describes how to reconstruct mitochondrial cristae to achieve 3D imaging with high accuracy, high resolution, and high throughput.