Liaocheng University 1 article published in JoVE Biology An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation Chong Teng*1, Kaidi Lyu*1, Qianqian Li1, Nan Li2, Shanhua Lyu1, Yinglun Fan1 1College of Agriculture, Liaocheng University, 2College of Life Science, Liaocheng University Here, we provide the detailed protocol of a one-step transformation method mediated by Agrobacterium tumefaciens to produce composite plants.