Ecole Polytechnique Federale de Lausanne View Institution's Website 12 articles published in JoVE Bioengineering Subdural Soft Electrocorticography (ECoG) Array Implantation and Long-Term Cortical Recording in Minipigs Florian Fallegger*1, Alix Trouillet*1, Stéphanie P. Lacour1 1Laboratory for Soft Bioelectronic Interfaces, Neuro-X Institute, Ecole Polytechnique Fédérale de Lausanne Here, we present a method for the long-term performance and safety assessment of soft subdural electrode arrays in a minipig model, describing surgical method and tools, postoperative magnetic resonance imaging, electrophysiology of the auditory cortex, electrochemical properties of the implant, and postmortem immunochemistry. Bioengineering OnePot PURE Cell-Free System Laura Grasemann*1, Barbora Lavickova*1, M. Carolina Elizondo-Cantú1, Sebastian J. Maerkl1 1Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne We present a fast and cost-effective method to produce the recombinant PURE cell-free TX-TL system using standard laboratory equipment. Environment Flash Infrared Annealing for Perovskite Solar Cell Processing Pui Sha Victoria Ling1,2, Anders Hagfeldt2, Sandy Sanchez2 1Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, 2Laboratory of Photomolecular Science (LSPM), École Polytechnique Fédérale de Lausanne (EPFL) We describe a flash infrared annealing method used for the synthesis of perovskite and mesoscopic-TiO2 films. Annealing parameters are varied and optimized for processing on fluorine-doped tin oxide (FTO) glass and indium tin oxide-coated polyethylene terephthalate (ITO PET), subsequently giving devices power conversion efficiencies >20%. Environment Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales David Scheidweiler1,2, Pietro De Anna2, Tom J. Battin1, Hannes Peter1 1Stream Biofilm and Ecosystem Research Laboratory, École Polytechnique Fédérale de Lausanne, 2Institute of Earth Sciences, University of Lausanne, CH-1015 Breakthrough curves (BTCs) are efficient tools to study the transport of bacteria in porous media. Here we introduce tools based on fluidic devices in combination with microscopy and flow cytometric counting to obtain BTCs. Engineering Monovalent Cation Doping of CH3NH3PbI3 for Efficient Perovskite Solar Cells Mojtaba Abdi-Jalebi1, M. Ibrahim Dar2, Aditya Sadhanala1, Satyaprasad P. Senanayak1, Michael Grätzel2, Richard H. Friend1 1Cavendish Laboratory, University of Cambridge, 2Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne Here, we present a protocol to adjust the properties of solution-processed CH3NH3PbI3 through the incorporation of monovalent cation additives in order to achieve highly efficient perovskite solar cells. Bioengineering Harmonic Nanoparticles for Regenerative Research Flavio Ronzoni1, Thibaud Magouroux2, Remi Vernet1, Jérôme Extermann3, Darragh Crotty4, Adriele Prina-Mello5, Daniel Ciepielewski6, Yuri Volkov4, Luigi Bonacina2, Jean-Pierre Wolf2, Marisa Jaconi1 1Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, 2Physics Department, GAP-Biophotonics, University of Geneva, 3Laboratoire d'Optique Biomédicale (LOB), Faculté des Sciences et Techniques de l'Ingénieur, École Polytechnique Fédérale de Lausanne, 4Department of Clinical Medicine, School of Medicine, Trinity College Dublin, 5School of Medicine and CRANN, Trinity College Dublin, 6Nikon AG Instruments Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented. Bioengineering Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy Esra Güç*1, Manuel Fankhauser*1, Amanda W. Lund1,2, Melody A. Swartz1, Witold W. Kilarski1 1Institute of Bioengineering and Swiss Institute of Experimental Cancer Research (ISREC), École Polytechnique Fédérale de Lausanne, 2Department of Cell and Developmental Biology and Knight Cancer Institute, Oregon Health & Science University The extracellular matrix undergoes substantial remodeling during wound healing, inflammation and tumorigenesis. We present a novel intravital immunofluorescence microscopy approach to visualize the dynamics of fibrillar as well as mesh-like matrix components with high spatial and temporal resolution using epifluorescence or two-photon microscopy. Engineering Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting Corsin Battaglia1,2, Karin Söderström1, Jordi Escarré1, Franz-Josef Haug1, Matthieu Despeisse1, Christophe Ballif1 1Institute of Microengineering (IMT), Photovoltaics and Thin Film Electronics Laboratory, Ecole Polytechnique Fédérale de Lausanne (EPFL), 2Department of Electrical Engineering and Computer Sciences, University of California, Berkeley We describe a nanomoulding technique which allows low-cost nanoscale patterning of functional materials, materials stacks and full devices. Nanomoulding can be performed on any nanoimprinting setup and can be applied to a wide range of materials and deposition processes. Engineering Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures Sergey V. Borisenko1, Volodymyr B. Zabolotnyy1, Alexander A. Kordyuk1,2, Danil V. Evtushinsky1, Timur K. Kim1,3, Emanuela Carleschi4, Bryan P. Doyle4, Rosalba Fittipaldi5, Mario Cuoco5, Antonio Vecchione5, Helmut Berger6 1Institute for Solid State Research, IFW-Dresden, 2Institute of Metal Physics of National Academy of Sciences of Ukraine, 3Diamond Light Source LTD, 4Department of Physics, University of Johannesburg, 5CNR-SPIN, and Dipartimento di Fisica "E. R. Caianiello", Università di Salerno, 6Institute of Physics of Complex Matter, École Polytechnique Fédérale de Lausanne The overall goal of this method is to determine the low-energy electronic structure of solids at ultra-low temperatures using Angle-Resolved Photoemission Spectroscopy with synchrotron radiation. Immunology and Infection TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination Melanie Blokesch1 1Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL) A quick method to modify the genome of V. cholerae is described. These modifications include the deletion of single genes, gene clusters and genomic islands as well as the integration of short sequences (e.g. promoter elements or affinity-tag sequences). The method is based on the natural transformation and FLP-recombination. Medicine How to Measure Cortical Folding from MR Images: a Step-by-Step Tutorial to Compute Local Gyrification Index Marie Schaer1, Meritxell Bach Cuadra2,3, Nick Schmansky4, Bruce Fischl4, Jean-Philippe Thiran2, Stephan Eliez1 1Department of Psychiatry, University of Geneva School of Medicine, 2Signal Processing Laboratory, École Polytechnique Fédérale de Lausanne, 3Department of Radiology, University Hospital Center and University of Lausanne, 4Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital Measuring gyrification (cortical folding) at any age represents a window into early brain development. Hence, we previously developed an algorithm to measure local gyrification at thousands of points over the hemisphere1. In this paper, we detail the computation of this local gyrification index. Neuroscience Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue Graham Knott1, Stéphanie Rosset1, Marco Cantoni1 1Centre of interdisciplinary electron microscopy, École Polytechnique Fédérale de Lausanne This protocol describes how resin embedded brain tissue can be prepared and imaged in the three dimensions in the focussed ion beam, scanning electron microscope.