UVO3 Ltd. 1 article published in JoVE Biochemistry Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC Stuart W. McKellar1, Ivayla Ivanova1, Robert W. van Nues2, Ross A. Cordiner3, Mehak Chauhan1, Niki Christopoulou1, Will Worboys4, Andrew Langford4, Torben Heick Jensen3, Sander Granneman1 1Centre for Engineering Biology, University of Edinburgh, 2Institute of Cell Biology, University of Edinburgh, 3Department of Molecular Biology and Genetics, Aarhus University, 4UVO3 Ltd. Kinetic cross-linking and analysis of cDNA is a method that allows investigation of the dynamics of protein-RNA interactions in living cells at high temporal resolution. Here the protocol is described in detail, including the growth of yeast cells, UV cross-linking, harvesting, protein purification, and next generation sequencing library preparation steps.