University of Missouri-Saint Louis 1 article published in JoVE Chemistry Versatile Technique to Produce a Hierarchical Design in Nanoporous Gold Palak Sondhi1, Dharmendra Neupane1, Jay K. Bhattarai2, Hafsah Ali1, Alexei V. Demchenko3, Keith J. Stine1 1Department of Chemistry and Biochemistry, University of Missouri-Saint Louis, 2Mallinckrodt Pharmaceuticals Company, 3Department of Chemistry, Saint Louis University Nanoporous gold with a hierarchical and bimodal pore size distribution can be produced by combining electrochemical and chemical dealloying. The composition of the alloy can be monitored via EDS-SEM examination as the dealloying process advances. The material's loading capacity can be determined by studying protein adsorption onto the material.