Protocol
Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
Florence A Giger1, Julien G Dumortier2, Nicolas B David1
1CNRS UMR8197 – INSERM U1024, IBENS, Institut de Biologie de l'École Normale Supérieure, 2Department of Physiology Development and Neuroscience, University of Cambridge
Combining cell transplantation, cytoskeletal labeling and loss/gain of function approaches, this protocol describes how the migrating zebrafish prospective prechordal plate can be used to analyze the function of a candidate gene in in vivo cell migration.
Comparing the Frequency Effect Between the Lexical Decision and Naming Tasks in Chinese
Xin-Yu Gao*1, Meng-Feng Li*1, Tai-Li Chou*2, Jei-Tun Wu1
1Department of Psychology, National Taiwan University, 2Neurobiology and Cognitive Science Center, National Taiwan University
Researchers adopt both the lexical decision task and the naming task to investigate some important topics such as character/word recognition by comparing the frequency effect between these two tasks. This article introduces this approach through two exemplar experiments and elaborates on the underlying logic.
Development of an In Vitro Ocular Platform to Test Contact Lenses
Chau-Minh Phan1, Hendrik Walther1, Huayi Gao2, Jordan Rossy1, Lakshman N. Subbaraman1, Lyndon Jones1
1School of Optometry and Vision Science, University of Waterloo, 2Medella Health
Current in vitro models for evaluating contact lenses (CLs) and other eye-related applications are severely limited. The presented ocular platform simulates physiological tear flow, tear volume, air exposure and mechanical wear. This system is highly versatile and can be applied to various in vitro analyses with CLs.
Visualization of Twitching Motility and Characterization of the Role of the PilG in Xylella fastidiosa
Xiangyang Shi1, Hong Lin2
1Department of Plant Science, University of California, Davis, 2Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, United States Department of Agriculture
In this study, a nano-microfluidic flow chamber was employed to visualize and functionally characterize the twitching motility of Xylella fastidiosa, a bacterium that causes Pierce's disease in grapevine.
Disclosures
No conflicts of interest declared.