Cell Culture Imaging
Cell Culture Imaging - Related Videos
10:41
Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells
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Cited by 8 •
2014
Somitogenesis is a rhythmic developmental process that spatially patterns the body axis of vertebrate embryos. Previously, we developed transgenic zebrafish lines that use fluorescent reporters to observe the cyclic genes that drive this process. Here, we culture dispersed cells from these lines and image their oscillations over time in vitro.
09:30
Imaging Local Ca2+ Signals in Cultured Mammalian Cells
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Cited by 17 •
2015
Here we present techniques for imaging local IP3-mediated Ca2+ events using fluorescence microscopy in intact mammalian cells loaded with Ca2+ indicators together with an algorithm that automates identification and analysis of these events.
16:29
Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones
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Cited by 18 •
2008
Aplysia californica neurons develop large growth cones in culture that are excellent for high-resolution imaging of growth cone motility and guidance. Here, we present a protocol for dissection and plating of Aplysia bag cell neurons as well as for setting up a chamber for live cell imaging.
10:12
Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
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Cited by 2 •
2017
Live imaging is a powerful tool to study cellular behaviors in real time. Here, we describe a protocol for time-lapse video-microscopy of primary cerebral cortex cells that allows a detailed examination of the phases enacted during the lineage progression from primary neural stem cells to differentiated neurons and glia.
08:02
Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells
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Cited by 1 •
2025
Endocytic and retrograde transport of proteins from the plasma membrane to the trans-Golgi network is essential to maintaining membrane homeostasis and regulating signaling. Here, we describe a method to image and quantify endocytic transport of transmembrane cargo proteins by live-cell microscopy using derivatized anti-GFP nanobodies in HeLa cells.
FAQs
Bright-field and phase-contrast methods reveal cellular appearance using optical contrast, while fluorescence highlights selected cellular features through fluorescent signals. Time-lapse imaging adds repeated observations, allowing researchers to follow changes in morphology, growth, and behavior. Selecting among these approaches depends on whether the experiment prioritizes general structure, specific features, or cellular dynamics.
Cell morphology can act as a visible indicator of how cells respond to their surroundings. By converting observed features into quantitative measurements, researchers can compare cellular states across biomaterials, tissue-engineered constructs, organoids, or experimental conditions. These comparisons help connect a visual phenotype with a mechanical or chemical cue and support more informed system design.
Repeated imaging shows how cellular features change rather than capturing only one state. This makes it possible to monitor growth, behavior, and responses over time while cells remain in controlled culture environments. In bioengineering studies, longitudinal observations can help identify how an engineered system evolves and whether its cellular response remains consistent during the experiment.
Imaging provides a way to compare visible cellular responses under different experimental conditions. Changes in morphology, growth, behavior, or viability-related observations can be evaluated alongside the mechanical or chemical cues applied to the culture. This linkage helps researchers determine how the engineered environment influences cells and guides the design of more physiologically relevant systems.
Researchers maintain cells under controlled laboratory conditions, select an optical imaging approach, and capture cellular features at appropriate observation points or across a time course. The resulting images are then analyzed to convert visual observations into quantitative measurements. Those measurements can be compared with the tested culture condition, material, construct, or cue to evaluate the system.
The approach is useful when researchers need to evaluate how cells interact with or develop within an engineered environment. Imaging can support assessment of biomaterials, tissue-engineered constructs, and organoid development by documenting cellular morphology, growth, behavior, and responses over time. These observations provide evidence for refining the design and performance of the engineered system.
Quantitative image-based observations allow researchers to compare cellular outcomes across culture conditions and experimental runs. Monitoring morphology, growth, behavior, and responses can reveal whether a process produces the intended cellular state or requires adjustment. In this way, imaging contributes both to quality control and to optimization of engineered culture systems without relying only on endpoint judgments.