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Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
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Neuroscience
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JoVE Journal Neuroscience
Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

DOI:
10.3791/56063-v

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10:12 min

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August 09, 2017

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, , , ,
1Brain Institute,Federal University of Rio Grande do Norte, 2Department of Biosystems Science and Engineering,ETH Zurich, 3The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University

Chapters

  • 00:05Title
  • 00:57Dorsolateral Telencephalon Microdissection and Cell Plating
  • 04:23Time-lapse Video Microscopy
  • 05:24Cell Tracking
  • 08:12Results: Cell Lineage of Single Progenitor Cells
  • 09:41Conclusion

Summary

Automatic Translation

Automatic Translation

Live imaging is a powerful tool to study cellular behaviors in real time. Here, we describe a protocol for time-lapse video-microscopy of primary cerebral cortex cells that allows a detailed examination of the phases enacted during the lineage progression from primary neural stem cells to differentiated neurons and glia.

Tags

Keywords: Live ImagingPrimary Cerebral Cortex Cells2D Culture SystemNeural Stem CellsNeuronsGlial CellsNeurodevelopmentCell LineageCell DivisionCell CycleCell GrowthEmbryonic BrainDorsolateral TelencephalonCell DissociationTrypsin-EDTAProliferation MediumPoly-D-lysine

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