Fibroblast Expansion
Fibroblast Expansion - Related Videos
08:34
Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
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Cited by 11 •
2021
This protocol presents a device that produces PRP to boost the in vitro expansion of cells in a 100% autologous fibroblast culture system.
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
09:42
Analysis of Thermal Expansion via Dilatometry
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2023
Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a material may or may not be desirable, depending on the application. For instance, in a common liquid thermometer, a material with a high thermal expansion would be...
05:54
Rapid Culturing of Primary Murine Melanocytes and Fibroblasts: Isolating Melanocytes and Fibroblasts from Whole Murine Skin Sample
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2023
This video describes the rapid method to isolate and culture the melanocytes and fibroblast simultaneously from the postnatal mouse pup’s skin. These cells can be maintained and manipulated in vitro to study skin cell biology, wound healing, and melanoma.
07:12
Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures
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Cited by 3 •
2012
Despite ongoing efforts to transition cultures to feeder-free conditions, the derivation and culture of human embryonic stem cells (hESC) remain largely dependent on co-cultures with mouse embryonic feeders (MEFs). Here, we show a novel methodology for rapidly removing feeders from hESC cultures prior to experimentation.
FAQs
Surface attachment provides the physical basis for maintaining fibroblasts as an adherent culture, while available space affects whether the population can continue expanding. If cells become overcrowded, growth conditions become less controlled and the resulting population may be less suitable for reproducible experiments. Managing attachment and available surface area therefore supports consistent cell preparation for bioengineering studies.
During Fibroblast Expansion, nutrients, temperature, and gas conditions must remain suitable for cell growth. These variables work together rather than acting as interchangeable controls: a culture may have adequate space yet perform poorly if its environmental or nutritional support is inappropriate. Keeping them controlled helps maintain viable populations and improves the consistency of cells used in downstream experiments.
Passage history matters because repeated cycles of growth and transfer can influence how experimental cell populations are characterized and compared. Researchers therefore track passage history while expanding cells and passage them before overcrowding compromises culture management. This record supports reproducibility, especially when fibroblasts are used across multiple biomaterial, extracellular-matrix, or tissue-repair experiments.
A basic workflow begins by maintaining attached cells under suitable nutrient, temperature, and gas conditions. Researchers monitor available space and population growth, then periodically passage the culture to limit overcrowding. The cells can subsequently be expanded to the quantity required for a study, with passage history recorded to support consistent comparisons between experiments.
Expanded populations can be incorporated into engineered tissues, used to evaluate cell-material interactions, or studied in models of extracellular-matrix production and tissue repair. These applications make cell preparation a foundation for testing tissue-related outcomes. The appropriate use depends on the experimental question and the desired downstream model, while controlled culture conditions help produce suitable, reproducible cell populations.
They can reveal how fibroblasts interact with a material and support studies of extracellular-matrix production. In engineered-tissue and tissue-repair models, the cultures provide a cellular system for examining outcomes related to those processes. Maintaining suitable culture conditions and documenting passage history is important when interpreting differences, because preparation quality affects reproducibility.