Recombinant Cd47 Modification
Recombinant Cd47 Modification - Related Videos
09:29
Genetic Modification and Recombination of Salivary Gland Organ Cultures
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Cited by 20 •
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A technique to genetically manipulate epithelial cells within whole ex vivo cultured embryonic mouse submandibular glands (SMGs) using viral gene transfer is described. This method takes advantage of the innate ability of SMG epithelium and mesenchyme to spontaneously recombine after separation and infection of epithelial rudiments with adenoviral vectors.
08:56
Recombineering and Gene Targeting
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2023
One of the most widely used tools in modern biology is molecular cloning with restriction enzymes, which create compatible ends between DNA fragments that allow them to be joined together. However, this technique has certain restrictions that limit its applicability for large or complex DNA construct generation. A newer technique that addresses some of these shortcomings is recombineering, which modifies DNA using homologous recombination (HR), the exchange between different DNA molecules based...
04:07
Genetic Modification of Primary Human Keratinocytes: A Method for Genetically Manipulating Keratinocytes Using Recombinant Retroviruses
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This video describes a detailed protocol for producing genetically modified primary human keratinocytes recombinant retrovirus. This method can be used to manipulate gene expression in keratinocytes.
02:32
Histone Modification
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2020
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression. Acetylation The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
14:23
Recombineering Homologous Recombination Constructs in Drosophila
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Cited by 9 •
2013
Homologous recombination techniques greatly advance Drosophila genetics by enabling the creation of molecularly precise mutations. The recent adoption of recombineering allows one to manipulate large pieces of DNA and transform them into Drosophila6. The methods presented here combine these techniques to rapidly generate large homologous recombination vectors.
FAQs
Defined sequence mutations let researchers test whether particular CD47 regions influence binding to signal regulatory protein alpha, or SIRPα. Comparing engineered variants can separate effects caused by the protein sequence from those caused by expression level or functional presentation. These comparisons help connect molecular design with macrophage recognition and changes in phagocytosis.
Sequence, abundance, and surface presentation represent different experimental variables. Altering expression can examine how much CD47 is available, while changing functional presentation can test how its display affects interaction with SIRPα. Keeping these design options distinct helps researchers interpret whether an observed immune response reflects molecular binding, protein availability, or how the protein is presented.
The CD47-SIRPα interaction provides a molecular link between engineered CD47 and macrophage behavior. When researchers compare modified forms, they can examine how changes in this interaction relate to macrophage recognition and phagocytosis. This makes the pair useful for connecting protein-level modifications with cell-level immune outcomes in controlled research systems.
Introducing defined tags or fusion partners changes how engineered CD47 can be presented or incorporated into a study while preserving a deliberate design for comparison. Such modifications support protein production, assay development, and functional testing. Their value lies in enabling researchers to evaluate engineered formats alongside sequence or expression changes when studying CD47 activity.
A general workflow begins by selecting the desired CD47 sequence, expression change, tag, mutation, or fusion design. Researchers then use recombinant DNA methods to produce the engineered form in cells. The resulting material or cells can support protein production, assay development, and evaluation of SIRPα binding, macrophage recognition, or phagocytosis.
This approach is useful when a study requires a defined CD47 variant rather than an unmodified protein. Bioengineers can apply it to mechanistic studies, protein production, and assay development, then extend the findings to therapeutic design. It is especially relevant when researchers need to connect molecular engineering choices with immune-cell interactions or material-based presentation.
Engineered CD47 provides a way to evaluate designs that may influence how cells are recognized and engulfed by macrophages. In cancer immunotherapy and cell therapy research, investigators can compare modified forms in relation to SIRPα interaction and phagocytosis. The same design logic also informs CD47-based biomaterials, linking molecular presentation to broader immune-function studies.