Immune Cell Metabolism
Immune Cell Metabolism - Related Videos
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
07:09
Isolation of Adipose Tissue Immune Cells
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Cited by 75 •
2013
Adipose tissue (AT) is a site of intense immune cell activation and interaction. Almost all cells of the immune system are present in AT and their ratios are altered by obesity. Proper isolation, quantification, and characterization of AT immune cell populations are critical for understanding their role in immunometabolic disease.
05:07
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
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Cited by 30 •
2016
We have established a technique for the isolation, phenotypic characterization and functional analysis of immune cells from murine gingiva.
FAQs
These signals regulate nutrient uptake and the activity of glycolysis, oxidative phosphorylation, and fatty acid oxidation. The resulting metabolic adjustments help a cell redirect available nutrients toward immediate energy production or the molecular building blocks required for proliferation, cytokine production, and differentiation. Metabolic regulation therefore acts as part of the functional response to immune stimulation.
These pathways provide complementary metabolic options that immune cells can regulate as their needs change. Their activity supports energy generation and supplies molecular building blocks for cellular processes. Examining how the pathways are coordinated helps explain why stimulation can alter proliferation, cytokine production, or differentiation rather than producing identical responses in every immune-cell state.
Immune-cell metabolism changes across tissues and conditions because cells adjust nutrient use and metabolic pathway activity to their surroundings and functional demands. Those differences can shape how cells survive, become activated, produce cytokines, proliferate, or differentiate. Comparing metabolic programs across settings therefore helps connect local biological conditions with variation in immune responses.
A useful analysis can focus on changes in nutrient uptake, glycolysis, oxidative phosphorylation, and fatty acid oxidation after immune receptors or inflammatory signals are engaged. Researchers can then relate these metabolic changes to outcomes such as proliferation, cytokine production, and differentiation. This links cellular metabolism with measurable aspects of immune-cell behavior and function.
This area is particularly informative when researchers need to understand why immune responses differ during infection, inflammation, cancer, or autoimmunity. Metabolic programs can help explain changes in immune-cell activity across these conditions. They also provide a framework for investigating how altered nutrient use and pathway regulation contribute to disease-associated immune behavior.
Metabolic research can identify how nutrient uptake and energy-producing pathways support specific immune-cell activities, including cytokine production, proliferation, and differentiation. That knowledge supports studies of therapies designed to modify immune-cell activity. In biology, the approach connects cellular mechanisms with potential strategies for changing immune responses in infection, inflammation, cancer, or autoimmunity.