Non Parenchymal Fraction
Non Parenchymal Fraction - Related Videos
09:49
Isolation and Cannulation of Cerebral Parenchymal Arterioles
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Cited by 28 •
2016
This manuscript describes a simple and reproducible protocol for isolation of intracerebral arterioles (a group of blood vessels encompassing parenchymal arterioles, penetrating arterioles and pre-capillary arterioles) from mice, to be used in pressure myography, immunofluorescence, biochemistry, and molecular studies.
03:41
Preparing Cerebral Endothelial Tubes from Mouse Parenchymal Arterioles
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2025
This video demonstrates a method for isolating the endothelial tubes from mouse cerebral parenchyma. Through a combination of enzymatic digestion and mechanical dissociation, the process yields an intact tube with endothelial cells.
08:47
Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells
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Cited by 96 •
2016
A technique to isolate human hepatocytes and non-parenchymal liver cells from the same donor is described. The different liver cell types build the basis for functional liver models and tissue engineering. This new method aims to isolate liver cells in a high yield and viability.
10:09
Fractional Distillation
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2023
Source: Laboratory of Dr. Nicholas Leadbeater — University of Connecticut Distillation is perhaps the most common laboratory technique employed by chemists for the purification of organic liquids. Compounds in a mixture with different boiling points separate into individual components when the mixture is carefully distilled. The two main types of distillation are "simple distillation" and "fractional distillation", and both are widely used in organic chemistry laboratories. Simple distillation...
11:10
Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice
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Cited by 3 •
2016
Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.
FAQs
Separation begins with tissue dissociation, which releases cells from the organ or tissue, followed by fractionation. Researchers can distinguish populations using physical or biological properties, including cell size, density, adhesion behavior, or cell-surface markers. The selected separation principle determines which supporting, vascular, immune, or stromal populations become available for subsequent biological analysis.
The fraction’s composition depends on how effectively tissue dissociation releases cells and which fractionation property is applied. Size, density, adhesion, and cell-surface markers can each divide populations differently. Consequently, the resulting material reflects both the tissue’s cellular organization and the separation strategy, which must be considered when interpreting cellular interactions or tissue remodeling.
This fraction provides access to cellular compartments that influence the behavior of primary functional cells without being the main parenchymal population. Examining these compartments helps researchers investigate signaling, inflammation, and remodeling within tissues. That perspective is particularly valuable when biological changes depend on communication between functional cells and their supporting, vascular, immune, or stromal surroundings.
A typical workflow starts with collecting the tissue or organ, dissociating it to release cells, and then applying a fractionation strategy. The separation may rely on size, density, adhesion, or cell-surface markers, depending on the populations of interest. The resulting fraction can then be examined to characterize cellular composition and related biological processes.
In liver research, analyzing this fraction helps examine cellular interactions among the organ’s functional and supporting compartments. Researchers can use the information to study inflammation, fibrosis, regeneration, and tissue remodeling. It also provides context for evaluating how drugs or other biological treatments affect cellular relationships and disease-related changes within the liver.
Analysis can reveal patterns relevant to signaling between cell populations, inflammatory activity, and remodeling of tissue structure. These observations support research into disease mechanisms, regenerative responses, fibrosis, and treatment effects. Rather than focusing only on primary functional cells, the approach broadens interpretation by showing how associated cellular compartments contribute to the tissue’s biological state.