Gastric Cancer Exosomes
Gastric Cancer Exosomes - Related Videos
04:21
Exosome Isolation: A Technique to Separate Exosomes from the Plasma of Non-small Cell Lung Cancer Patients
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2023
The plasma-isolated exosomes can be used as a non-invasive and repeatable way for the detection and follow-up of the biomarkers for early detection and disease progression. This video describes the protocol for the separation of exosomes from the plasma samples of non-small cell lung cancer patients, which could be used for DNA and protein extractions.
05:30
Laparoscopic Radical Gastrectomy for Remnant Gastric Cancer
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2025
Laparoscopic radical gastrectomy for remnant gastric cancer has been increasingly adopted as a minimally invasive surgical approach in recent years. This article proposes a detailed operating procedure, aiming to provide practical technical guidance for surgeons and promote the broader adoption of laparoscopic surgery in managing remnant gastric cancer.
03:43
Exosome Block Preparation: A Protocol to Preserve Exosomes From Cultured Human Colon Cancer Cells
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2023
This video describes the technique of preparing exosome blocks from cultured gastrointestinal tumor cells. These exosome blocks can further be sliced, stained, and imaged to determine the position of specific proteins in the lipid bilayer vesicle.
03:36
Gold Immunostaining of Exosomal Sections: A Technique to Visualize Diagnostic Markers in Exosomal Lumen
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2023
In this video, we demonstrate gold immunostaining of biomarkers localized within the lumen region of ultrathin exosomal sections. This technique enables early diagnosis of diseases by visualization of target molecules within the exosomes secreted by the diseased cells.
08:49
Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
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Cited by 59 •
2016
This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in NSCLC.
FAQs
Cargo carried by these vesicles can influence signaling, immune responses, invasion, and treatment sensitivity after reaching recipient cells. Proteins, lipids, messenger RNAs, and microRNAs provide different molecular inputs, allowing communication between tumor cells and surrounding cells. Studying these effects helps researchers connect exosome-mediated communication with changes in tumor progression and the tumor microenvironment.
The endosomal pathway provides the cellular route through which these vesicles form, while fusion of multivesicular bodies with the plasma membrane enables their release. This sequence links intracellular vesicle processing to extracellular communication. Examining it helps explain how gastric tumor or surrounding cells export molecular information that can subsequently influence other cells.
Uptake determines whether vesicle cargo can reach a recipient cell and produce a biological effect. In gastric cancer research, tracking this interaction helps clarify communication between tumor cells and the surrounding microenvironment. It can also connect transferred molecular information with altered signaling, immune behavior, invasion, or sensitivity to treatment.
Researchers examine proteins, lipids, messenger RNAs, and microRNAs within the vesicles because each cargo category may contribute to communication between cells. Linking cargo composition with recipient-cell responses can reveal how exosomes participate in signaling and tumor-related behavior. This approach supports investigation of mechanisms underlying progression and changes in treatment sensitivity.
Their molecular cargo offers measurable information about communication involving gastric tumor and surrounding cells. By analyzing that information, researchers can investigate whether exosome-associated signals reflect tumor progression or other cancer-related processes. The potential value of this approach is its relevance to minimally invasive biomarker development, although the overview identifies it as an area of investigation rather than an established clinical test.
Research examines these vesicles in two complementary ways: their cargo and communication effects may reveal therapeutic targets, while their natural capacity to transport molecular material supports investigation of exosome-based drug delivery. In gastric cancer, both strategies are intended to address tumor communication, the microenvironment, progression, or treatment sensitivity, but the overview presents them as research directions.