Targeted Dna Capture
Targeted Dna Capture - Related Videos
15:28
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
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Cited by 41 •
2009
We present a method of targeted ancient DNA sequence retrieval, which we used to reconstruct the complete mitochondrial genomes of five Neandertal individuals. Comparison of these sequences with present day humans suggests that Neandertals had a long term low effective population size.
06:26
Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
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Cited by 5 •
2012
Described is a two-step labeling process using β-glucosyltransferase (β-GT) to transfer an azide-glucose to 5-hmC, followed by click chemistry to transfer a biotin linker for easy and density-independent enrichment. This efficient and specific labeling method enables enrichment of 5-hmC with extremely low background and high-throughput epigenomic mapping via next-generation sequencing.
08:40
Methyl-binding DNA capture Sequencing for Patient Tissues
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Cited by 1 •
2016
Here we present a protocol to investigate genome wide DNA methylation in large scale clinical patient screening studies using the Methyl-Binding DNA Capture sequencing (MBDCap-seq or MBD-seq) technology and the subsequent bioinformatics analysis pipeline.
08:38
Targeted DNA Methylation Analysis by Next-generation Sequencing
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Cited by 83 •
2015
Bisulfite amplicon sequencing (BSAS) is a method for quantifying cytosine methylation in targeted genomic regions of interest. This method uses bisulfite conversion paired with PCR amplification of target regions prior to next-generation sequencing to produce absolute quantitation of DNA methylation at a base-specific level.
02:55
Immunoprecipitation of Target Protein-DNA Complexes from Motor Neuron Lysate
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2025
This video demonstrates the immunoprecipitation of DNA fragments crosslinked with target protein from stem cell-derived motor neuron lysates using target protein-specific antibody-coated magnetic beads.
FAQs
Complementary probe hybridization determines which genomic fragments are enriched. The oligonucleotide probes are designed to match selected DNA regions, so fragments containing those sequences can associate with the probes within a complex sample. This molecular recognition concentrates the regions chosen for study, allowing subsequent sequencing to focus on cancer-relevant genes or other predefined genomic targets.
Biotin-streptavidin magnetic beads provide the physical separation step. Probe-bound DNA can be associated with the bead system and then isolated from material that was not retained, producing an enriched fraction for sequencing preparation. This separation is important because the method depends not only on probe recognition, but also on recovering the selected fragments efficiently from the original DNA mixture.
Focusing sequencing on selected regions increases the proportion of reads directed toward informative loci rather than distributing effort across the entire genome. In cancer studies, that concentration can make it practical to examine genes or variants chosen in advance and supports analysis of mutations, copy-number changes, and other alterations. Findings remain centered on the regions included in the capture design.
A typical workflow begins by selecting the genomic regions to investigate and preparing probes complementary to those sequences. DNA from the sample is then exposed to the probes so matching fragments can hybridize. Probe-associated material is isolated with the bead-based system, and the enriched DNA is prepared for sequencing. The resulting data are interpreted within the predefined target set.
Captured regions can be examined in tumor DNA and, when available, compared with normal DNA. This comparison helps distinguish alterations associated with the tumor from sequence differences present in the normal sample. The resulting focused data can support characterization of cancer genomes and investigation of mechanisms linked to disease development, while keeping analysis centered on the selected genomic regions.
Targeted DNA capture is particularly useful when a project centers on a defined set of cancer genes or candidate regions. The enriched sequencing results can be used to investigate mutations and copy-number changes, examine other genomic alterations, and assess whether selected regions are relevant as biomarkers or potential therapeutic targets. It connects focused genomic measurement with specific cancer research questions.