Volumetric Pipette Technique
Volumetric Pipette Technique - Related Videos
11:43
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Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
08:08
Odorant-induced Responses Recorded from Olfactory Receptor Neurons using the Suction Pipette Technique
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Cited by 13 •
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Olfactory receptor neurons (ORNs) convert odor signals first into a receptor current that in turn triggers action potentials that are conveyed to second order neurons in the olfactory bulb. Here we describe the suction pipette technique to record simultaneously the odorant-induced receptor current and action potentials from mouse ORNs.
06:35
Introduction to Serological Pipettes and Pipettors
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2023
The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
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Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
06:26
Quantitative Assessment Protocol for Facial Soft Tissue Volumetric Changes with Stereophotogrammetry
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2025
We present a detailed protocol for three-dimensional analysis of soft tissue changes with the use of stereophotogrammetry.
FAQs
Eye-level reading prevents parallax, an apparent shift in the meniscus caused by viewing it from above or below. The operator adjusts the liquid until the bottom of the curved meniscus coincides with the calibration mark, then transfers the solution. This alignment is especially important when concentration calculations depend on the delivered volume.
Temperature control helps keep the pipette and solution at conditions consistent with the calibration. If temperature is not controlled, the intended volume may not correspond as closely to the calibrated volume, reducing the reliability of quantitative results. This consideration matters when preparing standard solutions or performing dilution calculations.
Gravity drainage and proper handling of the tip determine how much liquid reaches the receiving vessel. Touching the tip to the vessel wall helps release liquid clinging to the outlet, while the remaining film is not blown out. Consistent drainage practice supports reproducible transfers in quantitative chemistry.
Cleaning is an accuracy step, not merely a housekeeping task. A clean pipette supports reliable transfer of the intended solution and helps prevent conditions that could compromise concentration-dependent measurements. In chemistry, this is important before titrations, standard-solution preparation, and dilutions, where volume control directly affects the calculated result.
Before starting, confirm that the pipette is clean, the solution and pipette are under suitable temperature conditions, and a pipette filler is available for drawing liquid. During adjustment, place the calibration mark at eye level and align the bottom of the meniscus carefully. These checks reduce avoidable variation during quantitative work.
In titrations, the technique transfers a controlled amount of solution so the measured volume can be used in quantitative analysis. Accurate meniscus alignment, consistent gravity drainage, and correct tip handling help make that volume reproducible. The resulting measurement supports calculations that depend on the relationship between solution concentration and delivered volume.
Standard-solution preparation and dilution calculations require dependable volume measurements because the transferred amount determines the resulting concentration. Volumetric pipetting contributes to that control through careful calibration-mark reading, temperature awareness, cleaning, and consistent drainage. These practices help produce concentration-dependent measurements that are suitable for subsequent chemical analysis.