Human Colonic Organoids
Human Colonic Organoids - Related Videos
07:33
In vitro Organoid Culture of Primary Mouse Colon Tumors
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Cited by 44 •
2013
A simple method to establish primary murine colon tumor organoid is described. This method utilizes the feature that colon tumor cells survive and grow into organoids in media containing limited growth factors, whereas normal colon epithelial do not.
10:29
Generation and Genetic Manipulation of Human Cervical Organoids
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2026
We present a detailed protocol to establish and genetically manipulate human cervical organoids.
06:59
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
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Cited by 43 •
2013
The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.
10:30
Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
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Cited by 144 •
2015
Stem cell derived cultures harbor tremendous potential to model infectious diseases. Here, the culture of mouse and human gastric organoids derived from adult stem cells is described. The organoids are microinjected with the gastric pathogen Helicobacter pylori.
03:33
An In Vitro Method to Generate Human Brain Organoids
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2025
The video demonstrates a method for generating human brain organoids from induced pluripotent stem cells. It shows how the neurosphere forms a neuroectodermal layer, which suppresses the formation of other germ layers. The neurospheres are embedded in a 3D gel matrix and form neuroepithelial buds. When incubated with organoid media, these neuroepithelial buds differentiate into specific brain regions, resulting in the formation of mature brain organoids.
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These signals form a defined regulatory environment that controls whether intestinal stem cells continue self-renewing, differentiate into epithelial lineages, and organize within the extracellular matrix. Adjusting the balance of Wnt, R-spondin, EGF, and BMP pathway activity therefore influences tissue organization and cell fate. This makes the system useful for testing how developmental signals coordinate colonic epithelial formation.
Crypt formation and epithelial maturation provide developmental indicators that the cultured tissue is organizing and acquiring specialized epithelial characteristics. Examining these outcomes allows researchers to connect signaling conditions with structural development and cell-fate progression. The same readouts can help reveal congenital or disease-associated defects that alter normal colonic development.
They provide an experimentally accessible setting in which researchers can examine human epithelial development under controlled conditions. Because stem-cell renewal, differentiation, tissue organization, crypt formation, and maturation can be studied together, the cultures help connect an observed defect with a developmental process. This supports investigation of congenital abnormalities and disease-associated changes in the colon.
The cultures require intestinal stem cells embedded in a defined extracellular matrix and exposed to controlled developmental signals. Wnt, R-spondin, and EGF signaling, together with BMP pathway modulation, supports the coordinated processes of stem-cell self-renewal, epithelial differentiation, and tissue organization. Researchers can use these defined conditions to examine how changing regulatory inputs affects organoid development.
Their organized human colonic epithelium provides a model system for examining interactions between host tissue and microbes. Researchers can investigate these relationships in the context of epithelial structure, maturation, and tissue behavior rather than studying isolated cells alone. This application extends developmental modeling toward questions about how microbial exposure relates to colonic epithelial function and organization.
Patient-specific cultures can support studies of tissue behavior that reflects an individual biological background. They are also used to examine responses to drugs, allowing researchers to relate treatment effects to human colonic tissue characteristics. Combined with developmental analyses, these models can connect patient-associated defects or behaviors with epithelial organization, maturation, and signaling-dependent processes.