Single Track Deposition

Single track deposition is a materials-processing technique in which one continuous line of material is deposited onto a substrate, providing a controlled way to study deposition behavior. During the process, a moving heat source melts powder or wire feedstock and forms a localized melt pool; its flow, solidification, and bonding to the substrate determine the track’s geometry and quality. In engineering, single track deposition is widely used to evaluate process parameters, including energy input, travel speed, and feed rate, before producing multilayer parts. Measurements of bead shape, dilution, porosity, and defects help optimize directed energy deposition and related additive-manufacturing processes.

Single Track Deposition - Related Videos

Research

JoVE Journal - Engineering

Production of Single Tracks of Ti-6Al-4V by Directed Energy Deposition to Determine the Layer Thickness for Multilayer Deposition

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Cited by 39 •

2018

In this research, a rapid method based on melt pool characterization is developed to estimate the layer thickness of Ti-6Al-4V components produced by directed energy deposition.

Research

JoVE Journal - Biochemistry
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Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

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2025

This article provides a detailed description of how to create samples for single-protein tracking in solid-supported lipid bilayers. It also explains a straightforward fluorescence microscope with single-molecule sensitivity and a fast frame rate. Finally, we outline the procedure for extracting single-protein trajectories.

Tracking Bacterial Growth at Single-Cell Resolution in a Microfluidic System

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2025

Source: Cabeen, M. T., et al. Single-cell Microfluidic Analysis of Bacillus subtilis. J. Vis. Exp. (2018)The video demonstrates the use of a microfluidic device to observe bacterial growth at the single-cell level. It showcases how non-motile bacterial cells, confined within micro-scale trenches, are continuously nourished and imaged under a fluorescence microscope. The setup enables real-time visualization of cell division and lineage tracking across multiple generations.

Simultaneous Eye Tracking and Single-Neuron Recording to Identify Target-Selective Neurons

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2025

The video demonstrates an assay to identify target-selective neurons via simultaneous eye-tracking and single-neuron recordings. A human participant is tasked with identifying a target object among an array of objects in a display, and the eye movement is tracked. Simultaneously, single neurons' firing rate is recorded via electrodes implanted in the brain. The data from both recordings are correlated to assess the presence of target-selective neurons.

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

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Cited by 38 •

2014

Photoactivated localization microscopy (PALM) combined with single-molecule tracking allows direct observation and quantification of protein-DNA interactions in live Escherichia coli cells.

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