Polycarbonate Oak Ridge Tube
Polycarbonate Oak Ridge Tube - Related Videos
10:24
Neutron Radiography and Computed Tomography of Biological Systems at the Oak Ridge National Laboratory's High Flux Isotope Reactor
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Cited by 6 •
2021
This manuscript describes a protocol for neutron radiography and computed tomography of biological samples using a High Flux Isotope Reactor (HFIR) CG-1D beamline to measure a metal implant in a rat femur, a mouse lung, and an herbaceous plant root/soil system.
02:36
Polycarbonate Ultracentrifuge Tube Re-Use in Proteomic Analyses of Extracellular Vesicles
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2024
A detailed protocol is provided for cleaning and re-using polycarbonate ultracentrifuge tubes to perform extracellular vesicle isolation suitable for proteomics experiments.
08:18
Construction Methods for Hybrid Polycarbonate-Silicon Microfluidic Devices
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2026
This protocol describes three straightforward sealing methods for fabricating a hybrid microfluidic device that integrates polycarbonate and silicon components. By implementing solvent bonding, thermal compression, and mechanical compression strategies, we enable reliable, low-temperature sealing techniques that avoid specialized equipment for hybrid microfluidic devices.
07:37
Neural Tube Closure in Mouse Whole Embryo Culture
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Cited by 22 •
2011
A method allowing for direct pharmacological manipulation of mouse embryos during neurulation that bypasses maternal metabolism is described. The technique can be adapted to study different aspects of neurulation by varying the time point and pharmacological agent.
11:23
Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients
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Cited by 13 •
2017
The control of chemical and oxygen gradients is essential for cell cultures. This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) microfluidic device capable of reliably generating combinations of chemical and oxygen gradients for cell migration studies, which can be practically utilized in biological labs without sophisticated instrumentation.
FAQs
During centrifugation, centrifugal force causes suspended material to move relative to the surrounding liquid, allowing particles to sediment or separate when a density gradient is used. The tube’s thick walls maintain the sample container’s structure under these demanding conditions. This supports recovery of concentrated or fractionated biological material for subsequent immunology or infection-related analysis.
The screw cap helps contain liquids and aerosols while samples are subjected to high-speed centrifugation. This containment is important when workflows handle cells, microorganisms, viruses, or other biological material that must remain inside the tube during sedimentation or separation. Maintaining containment supports cleaner sample handling before downstream analysis in infection and immunology research.
Tube performance depends on compatibility with the centrifuge rotor, the solvent in the sample, the operating temperature, and the selected speed. Confirming these conditions helps ensure that the tube is used within an appropriate centrifugation setup. This check is particularly important for demanding workflows that apply substantial centrifugal force to biological samples.
Density-gradient centrifugation can separate biological material according to how particles behave within the gradient, while washing steps help prepare the collected material for later work. Using the same robust tube format for concentration, separation, and washing can support sequential sample preparation. These operations are relevant when immunology or infection studies require cleaner or more concentrated material.
A practical workflow begins by placing the biological sample in a compatible tube, selecting a rotor and operating conditions suited to the setup, and centrifuging to concentrate or separate the material. Researchers can then retain the relevant fraction or perform washing before downstream analysis. Compatibility with the solvent, temperature, and speed should be confirmed throughout the procedure.
These tubes support preparation of cells, microorganisms, viruses, and other biological material before downstream analysis. Depending on the experiment, centrifugation may concentrate material, separate components through a density gradient, or provide a washing step. Their rigid construction and sample-containment features make them useful for demanding preparation workflows connected to immunology and infection studies.