Murine Dissection
Murine Dissection - Related Videos
08:49
Murine Prostate Micro-dissection and Surgical Castration
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Cited by 23 •
2016
This manuscript describes the protocols for prostate micro-dissection and surgical castration in the laboratory mouse. We also depict representative results produced by these protocols. Finally, we discuss the advantages and utilization of these protocols.
07:50
Location, Dissection, and Analysis of the Murine Stellate Ganglion
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Cited by 15 •
2020
Pathophysiological changes in the cardiac autonomic nervous system, especially in its sympathetic branch, contribute to the onset and maintenance of ventricular arrhythmias. In the present protocol, we show how to characterize murine stellate ganglia to improve the understanding of the underlying molecular and cellular processes.
07:17
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
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Cited by 293 •
2016
Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.
02:16
A Dissection Method to Harvest Murine Popliteal Draining Lymph Nodes for Ex Vivo Imaging
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2025
This video demonstrates a dissection technique to harvest popliteal draining lymph nodes from the mouse hind legs. Lymph nodes play a critical role in bridging innate and adaptive immunity, and harvesting intact lymph nodes — with a preserved tissue structure and cellular viability — can help study local and systemic immune responses.
08:00
Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions
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Cited by 4 •
2020
Here we present a protocol for in vitro isolation of multiple glial cell populations from a mouse CNS. This method allows for the segregation of regional microglia, oligodendrocyte precursor cells, and astrocytes to study the phenotypes of each in a variety of culture systems.
FAQs
Sequential exposure helps preserve anatomical relationships before structures are reflected or removed. This allows observers to examine how organs and tissues are arranged in relation to one another rather than viewing isolated parts alone. Maintaining that sequence also supports later sampling and improves interpretation when gross anatomical findings must be compared with histology or other experimental results.
Gross examination records visible structural features, while histology can characterize tissue organization at a finer scale. Physiology and experimental findings then provide functional or treatment-related context. Comparing these levels helps investigators relate an observed anatomical change to tissue findings and possible medical significance, rather than interpreting the appearance of an organ without supporting biological context.
Careful positioning, controlled incisions, and deliberate reflection or removal of tissues influence whether structures remain identifiable. Approved animal-care and biosafety procedures also frame how the examination is conducted. Together, these considerations affect the preservation of anatomical relationships and the reliability of observations, sampling, and subsequent analysis in educational, pathological, developmental, or disease-model work.
The workflow begins with specimen positioning under approved animal-care and biosafety procedures. The skin is then incised, followed by the body wall, and tissues are exposed in sequence. Structures may be reflected or removed as needed for observation or sampling. Working systematically helps preserve relevant anatomy and supports a clearer record of findings.
Medical researchers may use this approach for pathology assessment, developmental studies, and evaluation of disease models or treatment effects. Examination can reveal structural changes associated with an experimental condition, while sampling supports additional analysis. Its value lies in connecting visible anatomy with broader biomedical questions about disease progression, development, or response to treatment.
Sampling extends the examination beyond visual inspection by making tissues available for further analysis. When samples are interpreted alongside gross anatomical observations, histology, physiology, or experimental findings, they can help clarify the significance of structural changes. This combined evidence supports more informed assessment of pathology, development, disease models, and treatment-related effects.