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Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

DOI:
10.3791/61345-v

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08:00 min

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June 04, 2020

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,
1Department of Neurosciences,Lerner Research Institute, Cleveland Clinic Foundation, 2Brain Health Research Institute,Kent State University

Chapters

  • 00:05Introduction
  • 00:38Cortex, Cerebellum, Brainstem, and Spinal Cord Dissection
  • 02:51Tissue Dissociation and Oligodendrocyte Precursor Cell Isolation
  • 06:00Results: OPC Differentiation in IFNγR-/- Mice
  • 07:31Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol for in vitro isolation of multiple glial cell populations from a mouse CNS. This method allows for the segregation of regional microglia, oligodendrocyte precursor cells, and astrocytes to study the phenotypes of each in a variety of culture systems.

Tags

Keywords: Murine GliaCentral Nervous SystemGlia HeterogeneityGlia IsolationTrypsinDNase ICell SuspensionRegional Differences

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