Basal Ganglia Injection
Basal Ganglia Injection - Related Videos
09:28
A Method of Nodose Ganglia Injection in Sprague-Dawley Rat
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Cited by 12 •
2014
Afferent vagal signaling transmits important information to central nervous system from receptors located in organs of the abdomen and thorax. The nodose ganglia of vagus nerves contain many types of receptors that modulate vagal activity. This protocol describes a method of local injections of neurochemicals into the nodose ganglia.
04:01
Isolation of Cells from Sensory Ganglia
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2025
This video demonstrates the isolation of cells from the sensory ganglia of mice. Ganglia are subjected to enzymatic digestion to dissolve the extracellular matrix. Single cells are then collected via filtering through a cell strainer followed by density gradient centrifugation for debris removal. Finally, washing and centrifugation are done to collect the isolated cells for future analysis.
09:11
Aplysia Ganglia Preparation for Electrophysiological and Molecular Analyses of Single Neurons
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Cited by 9 •
2014
Marine snail Aplysia californica has been widely used as a neurobiology model for the studies on cellular and molecular basis of behavior. Here a methodology is described for exploring the nervous system of Aplysia for the electrophysiological and molecular analyses of single neurons of identified neural circuitry.
12:00
Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats
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Cited by 8 •
2016
This manuscript describes how to prepare intact dorsal root ganglia for patch clamp recordings. This preparation maintains the microenvironment for neurons and satellite glial cells, thus avoiding the phenotypic and functional changes seen using dissociated DRG neurons.
02:57
Analyzing Tooth Germ and Trigeminal Ganglia Interactions Using a Microfluidic Co-culture System
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2025
This video demonstrates the utilization of a microfluidic device to study the interactions between tooth germ pulp and trigeminal ganglia. It showcases the placement of tissues inside the microfluidic device and provides a detailed visualization of trigeminal neurite growth toward the tooth germ through the device's microgrooves. Additionally, it highlights the role of tooth-derived molecular factors in promoting or inhibiting neurite extension into the tooth germ pulp, which is crucial for...
FAQs
Stereotaxic coordinates guide the needle or cannula to a selected basal ganglia nucleus rather than relying on visual access to the tissue. Accurate positioning helps confine the reagent to the intended target, making subsequent circuit or behavioral findings easier to interpret. Coordinate-based placement is therefore central to connecting a manipulation with a specific neural structure.
The injected reagent determines the type of information the experiment can provide. Tracers can support circuit mapping, whereas pharmacological agents or viral vectors can support targeted manipulation of neuronal activity. Because these substances serve different experimental purposes, the same stereotaxic placement can investigate either anatomical connectivity or functional contributions within basal ganglia circuits.
A controlled injection volume helps regulate how much experimental reagent is deposited within local tissue. This control is important because interpretation depends on associating the observed effect with the selected nucleus and reagent, rather than with an imprecisely distributed deposit. Volume control therefore supports more consistent comparisons between experimental conditions and strengthens conclusions about circuit function.
The procedure begins by using brain coordinates to plan the target location. An injection needle or cannula is then positioned stereotaxically at a nucleus such as the striatum, globus pallidus, or substantia nigra. The selected tracer, pharmacological agent, viral vector, or other reagent is released in a controlled volume so it can act within the local tissue.
The striatum, globus pallidus, and substantia nigra are among the nuclei that can be targeted. Selecting one of these locations allows investigators to examine localized contributions within basal ganglia circuits. Depending on the reagent and experimental design, the resulting study may address movement, learning, reward, neural connectivity, or mechanisms relevant to neurological disorders.
Verification confirms whether the deposited reagent reached the intended basal ganglia nucleus and remained appropriately localized. This step is essential because conclusions about neural circuits or behavior depend on the actual placement, not only the planned coordinates. If targeting is inaccurate, attributing an observed outcome to the intended structure becomes difficult, weakening interpretation of the experiment.