Calcium Triggered Swelling
Calcium Triggered Swelling - Related Videos
05:53
Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay
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Cited by 11 •
2018
This protocol aims to describe a method to examine the Ca2+ retention capacity and Ca2+- triggered mitochondrial swelling of isolated mitochondria of SH-SY5Y cells step-by-step.
07:04
Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients
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Cited by 121 •
2017
This protocol describes an assay for measuring CFTR function and CFTR modulator responses in cultured tissue from subjects with cystic fibrosis (CF). Biopsy-derived intestinal organoids swell in a cAMP-driven fashion, a response that is defective (or strongly reduced) in CF organoids and can be restored by exposure to CFTR modulators.
03:39
In Vivo Calcium Imaging Using Synaptically Localized Calcium Sensors in Drosophila melanogaster
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2025
This video demonstrates a method for in vivo calcium imaging using synaptically localized calcium sensors in flies. It outlines the steps for preparing the flies for microscopy, conducting olfactory conditioning, and visualizing odor-induced calcium influx.
07:33
Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS
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Cited by 10 •
2011
An efficient method to gain insights into visualizing the paracrine-derived ROS induction of endothelial Ca2+ signaling is described. This method takes advantage of measuring paracrine derived ROS triggered Ca2+ mobilization in vascular endothelial cells in a co-culture model.
11:06
In vivo Neuronal Calcium Imaging in C. elegans
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Cited by 20 •
2013
With its small transparent body, well-documented neuroanatomy and a host of amenable genetic techniques and reagents, C. elegans makes an ideal model organism for in vivo neuronal imaging using relatively simple, low-cost techniques. Here we describe single neuron imaging within intact adult animals using genetically encoded fluorescent calcium indicators.
FAQs
Excessive calcium entry can disturb the ion transport processes that normally help maintain cellular osmotic balance. As ion handling becomes disrupted, water accumulates inside the neuron and cell volume increases. This sequence links calcium dysregulation to physical swelling rather than treating swelling as an isolated structural event, helping explain how ionic disturbances contribute to neuronal injury.
Excitotoxicity involves conditions in which calcium regulation becomes harmful to neurons. Calcium-triggered swelling provides a structural consequence of that dysregulation: altered ion transport promotes water entry, increasing cell volume and potentially impairing membranes and organelles. Examining this connection helps researchers relate calcium imbalance to the loss of neuronal structural integrity associated with excitotoxic injury.
As intracellular water accumulation increases neuronal volume, the resulting structural change can interfere with membrane and organelle function. These effects matter because membranes and organelles support the cell’s organization and normal activity. Calcium-triggered swelling therefore serves as an indicator of more extensive cellular stress, linking changes in volume with deterioration of neuronal structure.
A study can relate the rise in intracellular calcium to changes in ion transport, osmotic balance, cell volume, and structural integrity. Considering these features together helps distinguish the initiating calcium disturbance from its downstream swelling response. This framework also allows researchers to assess whether a mechanism or intervention is associated with reduced cellular damage.
The process is relevant to excitotoxicity, ischemia, traumatic brain injury, and other conditions involving calcium dysregulation. Across these settings, researchers can examine whether excessive calcium entry accompanies altered ion handling, water accumulation, and impaired neuronal structure. Comparing these contexts helps clarify how calcium-related cellular injury contributes to nervous-system damage.
Researchers can use changes in calcium regulation, cell volume, membrane function, organelle function, and structural integrity as related outcomes when evaluating an intervention. An approach that limits calcium dysregulation or its effects on osmotic balance may reduce swelling and associated damage. This makes the process useful for investigating mechanisms that could help preserve neuronal integrity.