Esophageal Cannulation
Esophageal Cannulation - Related Videos
04:19
Robotic Enucleation of Esophageal Leiomyoma
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2026
This manuscript describes the surgical technique for robotic enucleation of an esophageal leiomyoma, serving as a reference for similar cases.
04:43
Esophageal 3D Raft Culture Model: An In Vitro Culture Technique to Generate Human Esophageal Mucosa Model
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2023
This video describes an in vitro culture technique to generate human organotypic esophageal constructs by co-culturing primary human esophageal fibroblast and squamous epithelial cells within a decellularized porcine scaffold. These 3D culture models mimic the function and physiology of their in vivo tissue counterparts.
02:05
Intraduodenal Cannulation in a Rat Model for Immunopathology Studies
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2025
This video demonstrates intraduodenal cannulation in a rat model. After anesthetizing the rat, the duodenum is located and a small puncture is created. A J-shaped cannula with a beveled tip is carefully inserted and secured, and the incision is closed. The cannulated rat model enables intra-intestinal infusion for immunopathology studies.
03:37
Intranasal Drug Administration in Mice via Pipette and Reverse-Cannulation for Nose-to-Brain Drug Delivery
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2025
Source: Kanazawa, T., et al. Novel Methods for Intranasal Administration Under Inhalation Anesthesia to Evaluate Nose-to-Brain Drug Delivery. J. Vis. Exp. (2018)This video demonstrates the intranasal administration of radiolabeled drugs in an anesthetized mouse to achieve nose-to-brain drug delivery. It explains how drugs cross the nasal epithelium and travel via neuronal pathways to reach the brain, as well as how ciliary motion influences drug retention and clearance. Additionally, the video...
05:18
Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
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Cited by 17 •
2011
A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.
FAQs
The cannula gives researchers controlled access to the esophageal lumen, so they can introduce defined volumes or selected stimuli rather than relying on uncontrolled delivery. This standardization reduces variation between experimental conditions and makes physiological responses easier to compare. As a result, investigators can more clearly relate esophageal stimulation to swallowing, distension, neural activity, or behavioral changes.
Esophageal cannulation can support studies of vagal signaling, visceral sensation, and communication between the gastrointestinal tract and nervous system. During or after stimulation, researchers may record responses such as swallowing, esophageal distension, or neural activity. Examining these responses helps connect events in the esophageal lumen with nervous-system processing and feeding-related behavior.
Controlled stimulation allows the gastrointestinal signal presented to an experimental subject to be defined more consistently. Researchers can then evaluate how that internal organ signal influences brain function, visceral sensation, and behavior. This is important for gut-brain research because it separates responses associated with the esophagus from variation caused by inconsistent delivery of substances or stimuli.
The technique provides access for introducing defined volumes or stimuli while researchers monitor outcomes such as swallowing, distension, and neural activity. Comparing the delivered condition with the recorded physiological response can help identify how esophageal input is represented. The resulting observations support analysis of both mechanical responses, such as distension, and associated neural or behavioral effects.
A typical workflow begins by placing a flexible cannula into the esophagus, followed by introducing a defined volume or stimulus through the tube. Researchers then monitor physiological or neural responses, which may include swallowing, distension, or neural activity. Keeping delivery controlled throughout this sequence helps produce interpretable measurements of esophageal signaling and its effects.
In neuroscience, Esophageal Cannulation can be applied to questions about vagal signaling, visceral sensation, feeding behavior, and gut-brain communication. It allows investigators to examine how controlled esophageal inputs relate to nervous-system activity and behavior. The method is therefore useful when the research goal is to connect a defined gastrointestinal event with physiological, neural, or behavioral outcomes.