In Vitro Preparation
In Vitro Preparation - Related Videos
08:16
Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates
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Cited by 6 •
2016
We demonstrate a method to generate 3D breast cancer surrogates, which can be cultured using a perfusion bioreactor system to deliver oxygen and nutrients. Following growth, surrogates are fixed and processed to paraffin for evaluation of parameters of interest. The evaluation of one such parameter, cell density, is explained.
06:36
PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP
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Cited by 35 •
2013
Implant-associated infection is a significant clinical complication. This study describes an approach using platelet-rich plasma (PRP) to prevent implant-associated infections, presents the protocol for preparing PRP with constant platelet concentration, and reports the newly identified antimicrobial properties of PRP and related protocols for examining such antimicrobial properties in vitro.
09:58
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
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Cited by 2 •
2017
This manuscript describes the efficient, non-viral delivery of miR to endothelial cells by a PEI/MNP vector and their magnetization. Thus, in addition to genetic modification, this approach allows for magnetic cell guidance and MRI detectability. The technique can be used to improve the characteristics of therapeutic cell products.
08:25
Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure
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Cited by 21 •
2020
This procedure was established to be used for developing advanced 3D hepatic cultures in vitro, which can provide a more physiologically relevant assessment of the genotoxic hazards associated with nanomaterial exposures over both an acute or long-term, repeated dose regimes.
08:07
Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments
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Cited by 6 •
2017
Here, we present the preparation of an er-xian decoction (EXD) in four steps—soaking, decoction, filtration, and concentration—and demonstrate the administration of a prepared EXD-containing serum to rats. These methods are applicable to the in vivo and in vitro study of herbal decoctions such as traditional Chinese medicines.
FAQs
Controlled temperature, oxygenation, ionic composition, and stimulation help determine whether neural tissue remains viable and whether its activity can be measured reliably. Adjusting these factors lets researchers examine neural responses under defined conditions rather than treating the preparation as an uncontrolled system. This precision is especially important when comparing synaptic transmission, neuronal excitability, or circuit function across experiments.
These supporting environments provide the conditions needed to maintain neural cells or tissue outside the organism. Nutrient-containing media support cultured neurons, whereas artificial extracellular solutions support preserved neural tissue during measurements. Selecting and controlling the surrounding solution helps sustain the preparation while allowing researchers to examine activity and responses to stimulation, drugs, or injury.
An isolated preparation can focus attention on synaptic transmission, neuronal excitability, and circuit function without the full complexity of a living organism. This separation makes it easier to relate a measured response to a defined experimental condition or stimulus. As a result, researchers can investigate neural mechanisms directly and evaluate how specific interventions alter activity.
Preparation viability and measurable activity depend on maintaining suitable temperature, oxygenation, and ionic composition, as well as applying stimulation in a controlled way. Changes in these conditions can affect how neural tissue or cultured neurons function and therefore influence experimental outcomes. Careful control allows observed differences to be interpreted as responses to the investigation rather than uncontrolled environmental changes.
A typical workflow begins with isolating or preparing neural cells, tissue, or an organ, followed by maintenance in nutrient-containing media or an artificial extracellular solution. Researchers then control temperature, oxygenation, ionic composition, and stimulation while recording activity or responses. The resulting preparation can support measurements of synaptic transmission, excitability, circuit function, drug effects, or injury responses.
Researchers choose this approach when they need direct access to neural tissue or cultured neurons and precise control over the experimental environment. It supports electrophysiology and imaging, enables testing of responses to drugs or injury, and helps model disease-related processes. The method is also useful for developing experimental and therapeutic strategies before addressing more complex biological settings.
By reducing the complexity present in a living organism, an in vitro preparation allows researchers to examine neural mechanisms under more precisely controlled conditions. Its findings can clarify how synaptic transmission, neuronal excitability, or circuit activity changes in response to defined manipulations. This focused information complements organism-level studies, which capture broader interactions but provide less experimental isolation.
Depending on the preparation and measurement, researchers can assess synaptic transmission, neuronal excitability, circuit function, and responses to stimulation, drugs, or injury. Electrophysiology can examine neural activity, while imaging can provide another way to observe responses. Together, these outcomes support disease modeling and the evaluation of experimental or therapeutic strategies under controlled laboratory conditions.