Intracellular Electrophysiology
Intracellular Electrophysiology - Related Videos
11:42
Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo
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Cited by 14 •
2016
Sharp microelectrodes enable accurate electrophysiological characterization of photoreceptor and visual interneuron output in living Drosophila. Here we show how to use this method to record high-quality voltage responses of individual cells to controlled light stimulation. This method is ideal for studying neural information processing in insect compound eyes.
10:46
Non-fluoroscopic Catheter Tracking for Fluoroscopy Reduction in Interventional Electrophysiology
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Cited by 4 •
2015
Radiation exposure is an underestimated risk in complex ablation procedures. Here, we describe a protocol to significantly decrease fluoroscopy time and dosage for both the patient and the lab staff by using a novel non-fluoroscopic catheter visualization system.
05:41
Applying Microfluidics to Electrophysiology
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Cited by 1 •
2007
Microfluidics can be integrated with standard electrophysiology techniques to allow new experimental modalities. Specifically, the motivation for the ...
09:51
Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia
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Cited by 4 •
2012
We demonstrate how changes in the intracellular free calcium concentration and synaptic efficacy can be simultaneously monitored in a ganglion preparation of Aplysia. We image intracellular calcium using a fluorescent dye, Calcium Orange, and induce and monitor synaptic transmission with sharp (intracellular) electrodes.
07:18
Intracellular Refolding Assay
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Cited by 9 •
2012
In this protocol a method to measure intracellular protein refolding after heat shock is described. This method can be used to study foldases like molecular chaperones and their co-factors or compounds able to influence their activity. Firefly luciferase activity is used as reporter to measure chaperone refolding activity.
FAQs
The measured variable depends on what the experiment controls and observes. Recording membrane voltage shows how the cell’s electrical state changes, whereas recording ionic current reveals movement associated with ion channel activity. Examining either variable, or relating the two under controlled conditions, helps researchers connect electrical signals with the cellular mechanisms that generate them.
Access to the cell interior allows investigators to measure electrical behavior directly at the level of an individual living cell. This makes it possible to examine resting membrane potential, action potential generation, synaptic responses, and ion channel activity within the same experimental framework. Such measurements connect cellular events with the signaling properties of neurons.
Changes in ion channel activity can alter the electrical behavior recorded from a neuron, including its membrane voltage or ionic current. Comparing these measurements across experimental conditions helps identify how cellular mechanisms contribute to signal generation and communication. This approach is especially useful when evaluating effects associated with pharmacological treatment or other changes in neuronal state.
Controlling experimental conditions helps researchers determine how a neuron responds to a defined stimulus or intervention rather than to uncontrolled changes in its environment. When voltage or ionic current is recorded while conditions and stimulation are managed, differences in the resulting signals can be interpreted more directly as changes in neuronal electrical function.
A typical experiment uses a fine microelectrode or patch-clamp pipette to access an individual living cell, establishes the desired recording configuration, and measures membrane voltage or ionic current. Researchers may also stimulate the cell while controlling relevant conditions. The resulting traces are then examined for electrical features such as action potentials, synaptic responses, or channel activity.
Neuroscientists use intracellular electrophysiology when they need cellular-level information about how an individual neuron generates or receives electrical signals. The technique can characterize neuronal function and circuit mechanisms, while also revealing changes linked to development, disease, or pharmacological treatment. Its value lies in connecting measured electrical properties with specific cellular and synaptic behaviors.