Nf-kb Activation
Nf-kb Activation - Related Videos
02:26
NF-κB-dependent Signaling Pathway
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2021
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals. NF-κB-dependent Signaling Mechanism The heterodimer of NF-κB...
10:57
NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
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Cited by 4 •
2020
Here, we present a protocol to quickly and easily measure nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation in cell lines expressing NF-κB::luciferase reporter constructs, via measurements of luminescence in the cell lysate. Additionally, gene expression is determined via RT-qPCR isolated from cells infected with Salmonella Typhimurium.
14:58
Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis
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Cited by 1 •
2014
Methods for the manipulation and analysis of NF-κB-dependent adult hippocampal neurogenesis are described. A detailed protocol is presented for a dentate gyrus-dependent behavioral test (termed the spatial pattern separation-Barnes maze) for the investigation of cognitive outcome in mice. This technique should also help enable investigations in other experimental settings.
08:51
Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic
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Cited by 14 •
2012
Described here is a technique in which lipopolysaccharide is injected into the lactating mouse mammary gland via the nipple to simulate mastitis, a condition commonly caused by bacterial infection. Lipopolysaccharide injection results in increased nuclear factor kappa B (NF-κB) signaling, visualized through bioluminescent imaging of an NF-κB luciferase reporter mouse.
09:30
Measurement of Larval Activity in the Drosophila Activity Monitor
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Cited by 10 •
2015
This report describes a method for measuring Drosophila larval activity using the TriKinetics Drosophila Activity Monitor. The device employs infrared beams to detect movements of up to 16 individual animals. Data can be analyzed to represent motion parameters including rates and the positions of the animals within the assay chambers.
FAQs
The IκB kinase complex acts as the pathway’s key control point. Once signaling activates it, the complex promotes IκB degradation. This removes the cytoplasmic inhibition that retains NF-κB, permitting nuclear entry and subsequent regulation of target genes. This sequence links an external stimulus to a transcriptional response.
Stress, infection, inflammation, and other stimuli are relevant initiating contexts for NF-κB signaling. Their importance is not limited to immune defense: in nervous-system research, the same activation framework is examined alongside microglial activation, neuroinflammation, neuronal survival, and synaptic function. Identifying the stimulus helps connect pathway activity with the biological response under study.
Its importance reflects the range of neural processes associated with the pathway. NF-κB activity is examined in relation to microglial activation and neuroinflammation, while also being linked with neuronal survival and synaptic function. This breadth allows researchers to investigate how one signaling system relates to immune, injury-related, and functional changes in the nervous system.
A useful conceptual sequence begins by comparing a resting cellular state with a state exposed to a relevant stimulus. Researchers can then examine activation of the IκB kinase complex, IκB degradation, NF-κB entry into the nucleus, and regulation of target genes. Following these linked stages helps determine where pathway activity changes during the response.
Following the pathway from kinase-complex activation through IκB degradation and nuclear NF-κB activity can connect an initiating signal with altered gene regulation. In neuroscience, that sequence can help relate signaling events to microglial activation, neuroinflammation, neuronal survival, or synaptic function. The resulting interpretation depends on which biological process is being investigated.
In neuroscience, researchers study this pathway to clarify mechanisms associated with brain injury and neurodegenerative disease. Its connection to neuroinflammation, microglial activation, neuronal survival, and synaptic function also makes it relevant when evaluating potential therapeutic strategies. The pathway therefore provides a molecular framework for relating cellular signaling to disease-related and functional changes in the brain.