Unfolded Hippocampus
Unfolded Hippocampus - Related Videos
03:25
Recording Neural Activity of Unfolded Hippocampal Tissue Using Penetrating Microelectrode Array
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2025
This video demonstrates the procedure to set up a recording chamber with a penetrating microelectrode array (PMEA) to capture neural signals from an unfolded hippocampus. This method allows us to study the speed and direction of propagation of neural activity within the unfolded hippocampus in vitro.
09:48
Neural Activity Propagation in an Unfolded Hippocampal Preparation with a Penetrating Micro-electrode Array
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Cited by 2 •
2015
We have developed an in vitro unfolded hippocampus which preserves CA1-CA3 array of neurons. Combined with the penetrating micro-electrode array, neural activity can be monitored in both the longitudinal and transverse orientations. This method provides advantages over hippocampal slice preparations as the propagation in the entire hippocampus can be recorded simultaneously.
01:37
The Unfolded Protein Response
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2023
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
02:38
Assessing Bacterial Chaperone Activity via Thermal Unfolding of a Model Protein
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2025
Source: Dahl, J. et al. Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo. J. Vis. Exp. (2016)This video demonstrates a fluorescence spectrophotometer-based assay to evaluate chaperone activity on a substrate protein under acid and heat stress. It outlines the steps for monitoring substrate unfolding and aggregation through light scattering, comparing conditions with and without the chaperone.
02:39
In Vitro Recording of Theta Oscillations in the Mouse Hippocampus
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2025
This video demonstrates the recording of rhythmic neuronal network activity, also known as theta oscillations, in an isolated hippocampus. Electrodes are inserted into various layers and spatial positions to detect and analyze theta oscillations. This shows the unique neuronal compositions and the connectivity of the neuronal network across different layers of the hippocampus.
FAQs
Flattening the hippocampal formation exposes its organization along anatomical axes, allowing the dentate gyrus, hippocampal fields, and associated cortical regions to be compared within one extended spatial layout. This view helps investigators inspect where structures lie relative to one another and trace connectivity patterns that are harder to interpret when curvature separates them across conventional sections.
The hippocampal formation is tightly curved, so neighboring regions may appear separated or differently oriented in standard views. Opening the tissue along its anatomical organization helps preserve the relationships among these regions in a more continuous arrangement. That perspective is important for examining how hippocampal circuitry is organized rather than interpreting each visible area in isolation.
Conventional sections provide selected views through the curved hippocampal formation, whereas the unfolded preparation supports inspection across a broader, flattened arrangement. Researchers can therefore evaluate lamellar organization, spatial relationships, and connectivity patterns together. The approach complements section-based analysis by making large-scale anatomical organization easier to visualize and compare.
The preparation supports joint examination of the dentate gyrus, hippocampal fields, and associated cortical regions. Considering these structures within the same anatomical layout helps researchers evaluate their relative positions and relationships across the hippocampal formation. This integrated view is useful when studying how regional organization contributes to broader hippocampal circuitry.
The preparation begins by separating or opening the tightly curved hippocampal tissue along its anatomical axes. Researchers then examine the resulting flattened organization to assess the placement of the dentate gyrus, hippocampal fields, and associated cortical regions. The key outcome is an anatomical view suited to analyzing spatial relationships and connectivity across the structure.
Researchers can use this preparation when they need to relate hippocampal anatomy to learning and memory, developmental changes, or neurological disease. Its broad spatial perspective helps reveal how organization and connectivity vary across the formation. It is especially valuable when curvature makes those relationships difficult to interpret in conventional anatomical views.
An unfolded hippocampus can provide evidence about lamellar organization, regional spatial relationships, and patterns of connectivity that support interpretation of hippocampal function. By linking these anatomical features with experimental measurements, investigators can examine how structural organization relates to learning, memory, development, or disease-related changes within a neuroscience research context.