Genetic Reporters
Genetic Reporters - Related Videos
08:50
Genetic Screens
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2023
Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then assessing the affected organisms for phenotypes of interest. The process can be “forward”, where mutations are generated randomly to identify unknown genes responsible for the phenotypes, or it can be “reverse”, where specific genes are targeted for mutation to observe what phenotypes are produced.Here, JoVE reviews various types of genetic...
09:06
An Introduction to Developmental Genetics
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2023
Development is the complex process through which a single-celled embryo transforms into a multicellular organism. Developmental processes are guided by information encoded in an organism's DNA, and geneticists are trying to understand how this information leads to a fully formed organism. This video reviews seminal research in the field of developmental biology, including the identification of specific genes that control various embryonic processes. An introduction to the major questions asked...
10:38
An Overview of Genetic Engineering
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2023
Genetic engineering – the process of purposefully altering an organism’s DNA – has been used to create powerful research tools and model organisms, and has also seen many agricultural applications. However, in order to engineer traits to tackle complex agricultural problems such as stress tolerance, or to realize the promise of gene therapy for treating human diseases, further advances in the field are still needed. Important considerations include the safe and efficient delivery of genetic...
11:40
Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
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Cited by 24 •
2020
Generation of recombinant rotaviruses from plasmid DNA provides an essential tool for the study of rotavirus replication and pathogenesis, and the development of rotavirus expression vectors and vaccines. Herein, we describe a simplified reverse genetics approach for generating recombinant rotaviruses, including strains expressing fluorescent reporter proteins.
05:45
In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
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Cited by 4 •
2019
Morphological changes occur in immune responsive fibroblast cells following activation and promote alterations in cellular recruitment. Utilizing 2-photon imaging in conjunction with a genetically engineered Fibroblast-specific protein 1 (FSP1)-cre; tdTomato floxed-stop-floxed (TB/TB) mouse line and green fluorescently tagged lipopolysaccharide-FITC, we can illustrate highly specific uptake of lipopolysaccharide in dermal fibroblasts and morphological changes in vivo.
FAQs
A reporter’s output depends on the promoter or other regulatory element used to control its reporter gene. When that regulatory control responds to a particular gene-expression program or cellular signal, the linked fluorescent, luminescent, or sensor output changes. This coupling lets the measured signal indicate biological activity under examination rather than serving as a generic label.
They can be configured around different signal types. A promoter or regulatory element can connect reporter output to gene expression, while an activity-sensitive sensor can report a changing cellular process such as calcium dynamics or circuit activity. The resulting color, brightness, or emitted light gives researchers a measurable readout suited to the biological event under study.
Fluorescent proteins produce changes in detectable color or brightness, whereas luminescent enzymes are monitored through emitted light. Both translate regulated biological events into measurable optical outputs, but the choice determines how the signal is represented and quantified. In neuroscience, either format can support visualization of labeled neuronal populations or activity-related changes in living cells.
Activity-sensitive sensors extend reporter use beyond identifying cells by linking output to functional events. They can reveal calcium dynamics or circuit activity in cultured cells and intact nervous systems, allowing molecularly engineered signals to be examined in relation to neuronal function. This helps connect cellular activity with broader questions about neural development, connectivity, and disease mechanisms.
Begin by selecting the gene-expression program or cellular signal to monitor, then pair an appropriate promoter or regulatory element with a reporter gene or activity-sensitive sensor. Researchers can examine resulting changes in color, brightness, or emitted light in living cells. This design links the chosen biological question to a measurable experimental output.
A regulatory element can associate reporter output with a particular pattern of gene regulation, making the resulting signal useful for labeling neuronal populations. In neuroscience, this approach supports identification of neuronal groups and examination of how their molecular identities relate to development or function in cultured cells and intact nervous systems.
They can reveal patterns of gene regulation during development while linking molecular events to cell identity and function. This combination helps investigators examine how neuronal populations relate to developing neural systems and study connections among development, connectivity, and functional activity. Measurements can be collected in cultured cells or intact nervous systems.
They are useful when researchers need to connect molecular changes with neural cell behavior or activity. Reporter-based measurements can support studies of disease mechanisms and responses to experimental treatments, while activity-related sensors can track calcium dynamics or circuit activity. These readouts help examine biological responses in cultured cells and intact nervous systems.