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Selektion og isolering Kolonier af Human induceret pluripotente stamceller omprogrammeret fra voksne fibroblaster

Article DOI: 10.3791/3416-v • 13:24 min • February 20th, 2012
February 20th, 2012 •
1,2, 1, 3, 3, 1, 1
1Department of Molecular Carcinogenesis and Center for Cancer Epigenetics, University of Texas M.D. Anderson Cancer Center, 2Department of Cell Biology, Poznan University of Medical Sciences, 3Department of Molecular Biology, The Scripps Research Institute

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Vi præsenterer en protokol til effektiv omprogrammering af humane somatiske celler i menneskelige inducerede pluripotente stamceller (hiPSC) ved hjælp af retrovirale vektorer koder Oct3 / 4, Sox2, Klf4 og c-myc (OSKM) og identifikation af korrekt omprogrammeres hiPSC af levende farvning med Tra- 1-81 antistof.

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Developmental Biology stamceller inducerede pluripotente stamceller IPSC somatisk celleterapi omprogrammering pluripotency retroviral transduktion
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