Journal
/
/
Discriminatie van zeven immuun cel deelverzamelingen door twee-fluorescerende Stroom Cytometry
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
DOI:

10:58 min

March 05, 2019

,

Chapters

  • 00:04Title
  • 00:32Cell Preparation
  • 02:15Cell Staining – General Procedure
  • 04:02Antibody Titration
  • 06:41Gating Strategy
  • 08:15Results: Analysis of Samples from Patients with Multiple Myeloma
  • 10:02Conclusion

Summary

자동 번역

Hier presenteren we een stroom cytometrische protocol ter identificatie van CD4+ en CD8+ T cellen en γδ T cellen, B-cellen, NK-cellen, monocyten in perifere bloed met behulp van slechts twee fluorochromes in plaats van zeven. Met deze aanpak, kunnen vijf extra markeringen op de meeste flow cytometers worden opgeslagen.

Related Videos

Read Article